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Abstract：
Diosmin (DIO) and hesperidin (HSP) are important classes of flavonoid glycoside effectively used to prevent comorbid diseases that are commonly associated with COVID-19. An innovative, green, ccurate, effective, cost, and timeless spectrophotometric strategy was established to analyze such challengeable mixture in a co-formulated tablet namely Diosed C® tablets that comprises DIO, HSP and vitamin C (VIT. C) in the ratio of (450 mg: 50 mg: 100 mg) necessary for prevention and treatment of COVID-19. Vitamin C was resolved through physical extraction using de-ionized water while DIO and HSP were extracted via spectrophotometric methods using two different solvents [0.1 M NaOH or solvent blend consisting of DMSO and methanol (1:1)]. Mathematical filtration technique is successfully applied to recover the parent spectra of both DIO and HSP via three methods which are absorbance resolution (AR), Induced absorbance resolution (IAR) and ratio extraction (RE). VIT. C was successfully analyzed in de-ionized water using its maxima at 266.0 nm in a linearity range 2.0-20.0 μg/mL, DIO was effectively determined in 0.1 M NaOH at 372.0 nm in a linearity range of 7.0-70.0 μg/mL as well as in solvent blend at 344.0 nm in linearity range of 5.0-55.0 μg/mL while HSP was accurately analyzed in 0.1 M NaOH at 240.0 nm in linearity range of 3.5-50.0 μg/mL as well as in solvent blend at 285.0 nm in linearity range of 4.0-50.0 μg/mL. Satisfactory results were accomplished when conducting ICH guidelines for assuring the methods validation. Comparative study was introduced in the analysis of such critical combination and was prosperously devoted for the effective analysis of pharmaceutical dosage form. The proposed extraction pathways undergo the guidelines of green analytical chemistry using Analytical Eco-Scale (AES), AGREE and GAPI greenness assessment tools which confirmed their eco-friendly nature with priority to 0.1 M NaOH. The obtained results of the suggested methods were set side by side with those of official/reported methods statistically and show satisfactory implications. The presented methods were simple, affordable, smoothly applicable and their results were acceptable that enhances their usage and application in the quality control laboratories.
摘要：
狄奥司明(DIO)和橙皮苷(HSP)是一类重要的类黄酮苷，可有效预防通常与COVID-19相关的合并症。一个创新的,绿色,ccurate,有效,成本,和永恒的分光光度策略被建立来分析这种具有挑战性的混合物在一个共同配制的片剂，即DiosedC®片剂，包括DIO，HSP和维生素C（VIT。C）以预防和治疗COVID-19所需的比例（450mg：50mg：100mg）。维生素C通过使用去离子水的物理提取来分解,而DIO和HSP通过分光光度法使用两种不同的溶剂[0.1MNaOH或由DMSO和甲醇(1:1)组成的溶剂混合物]来提取。通过三种方法成功地应用数学过滤技术来恢复DIO和HSP的母光谱，这三种方法是吸光度分辨率（AR），诱导吸光度分辨率(IAR)和比例提取(RE)。维生素。C在去离子水中使用其在266.0nm处的最大值在2.0-20.0μg/mL的线性范围内成功分析，在372.0nm的0.1MNaOH中，在7.0-70.0μg/mL的线性范围内，以及在344.0nm的溶剂混合物中，在5.0-55.0μg/mL的线性范围内，有效地测定了DIO，而在240.0nm的0.1MNaOH中，在3.5-50.0μg/mL的线性范围内，以及在285.0nm的溶剂混合物中，在4.0-50.0μg/mL的在进行ICH指南以确保方法验证时，获得了令人满意的结果。在这种关键组合的分析中引入了比较研究，并致力于药物剂型的有效分析。拟议的提取途径经过使用分析生态尺度（AES）的绿色分析化学指南，AGREE和GAPI绿色评估工具证实了其环保性质，优先考虑0.1MNaOH。建议方法的结果与官方/报告方法的结果在统计学上并排设置，并显示出令人满意的含义。提出的方法很简单，负担得起的,顺利适用，其结果是可以接受的，从而增强了其在质量控制实验室中的使用和应用。
