PDF(Pubmed)
Abstract：
Panwad (Cassia tora L.), Sarshaf (Brassica nigra L.) and Kunjad (Sesamum indicum L.) have been used in the treatment of ringworm infection since ancient times and are documented in classical literature of Unani Medicine. These plant drugs give promising results when used topically in the form of paste (zimad). Hence, the development and evaluation of a cream containing extracts of Tukhm-e-Panwad (Cassia tora L.), Sarshaf (Brassica nigra L.), and Kunjad (Sesamum indicum L.) was carried out to get better efficacy of the drugs. A total of 16 batches (F1-F16) of cream were prepared by adding varying concentrations of hydro-alcoholic extract of the drugs (20%, 40% and 50%) in water removable bases, and three batches were selected as final batches (F4-20%, F6-40% and F16-50%). In vitro antidermatophytic activity was conducted to optimize MIC against dermatophytosis-causing fungi. Dermal irritation of the prepared cream was determined in New Zealand Albino Rabbits. In vivo testing of the different concentrations of the prepared cream (20%, 40%, and 50%) was also carried out using Wistar rats to assess the antidermatophytic activity. Final batches showed good results in all the tested parameters and significant in vitro and in vivo antifungal activity in a dose-dependent manner. No microbial growth was seen in the prepared formulation. The study revealed significant antidermatophytic activity of the prepared cream against dermatophytosis-causing fungi. Hence, it can be concluded that the prepared cream can be an alternative topical agent with safe and effective antifungal activity for treating dermatophytosis.
摘要：
Panwad（决明子L.），Sarshaf（BrassicanigraL.）和Kunjad（SesamumindicumL.）自古以来就被用于治疗癣感染，并在Unani医学的古典文学中有记载。当以糊剂(zimad)的形式局部使用时,这些植物药物给出有希望的结果。因此，含有Tukhm-e-Panwad（决明子）提取物的乳膏的开发和评估，Sarshaf(BrassicanigraL.),并进行了Kunjad（SesamumindicumL.）以获得更好的药物疗效。通过添加不同浓度的药物水醇提取物（20％，40％和50％）在水中可移动底座中，并选择三个批次作为最终批次(F4-20%，F6-40%和F16-50%）。进行了体外抗皮肤癣菌活性，以优化对引起皮肤癣菌的真菌的MIC。在新西兰白化兔中测定制备的乳膏的皮肤刺激。不同浓度制备的乳膏的体内试验(20%,40%,和50％）还使用Wistar大鼠进行了评估，以评估其抗皮肤癣菌活性。最终批次在所有测试参数中都显示出良好的结果，并且以剂量依赖性方式在体外和体内具有显着的抗真菌活性。在制备的制剂中没有看到微生物生长。研究表明，制备的乳膏对引起皮肤癣菌的真菌具有显着的抗皮肤癣菌活性。因此，可以得出结论，所制备的乳膏可以作为治疗皮肤癣菌病的具有安全有效抗真菌活性的替代外用药物。
