PDF(Pubmed)
Abstract：
Pterygium is one of the most frequent pathologies in ophthalmology, and is a benign, overgrowth of fibrovascular tissue, often with a wing-like appearance, from the conjunctiva over the cornea. It is composed of an epithelium and highly vascular, sub-epithelial, loose connective tissue. There is much debate surround the pathogenesis of pterygium and a number of theories have been put forward including genetic instability, cellular proliferation, inflammatory influence, and degeneration of connective tissue, angiogenesis, aberrant apoptosis and viral infection. At present, the involvement of human papillomavirus (HPV) in the genesis of pterygium is controversial, as have reported that HPV is present in 58% of cases, while others have failed to detect HPV in pterygium. In this study, we evaluated the presence and viral genotype of HPV DNA in pterygia and healthy conjunctiva sample, and virus integration into the cellular genome. Forty primary pterygia samples and 12 healthy conjunctiva samples were analyzed to HPV DNA presence by polymerase chain reaction, using MY09/MY11 primers of HPV-L1 gene. Viral genotype was identified by DNA sequence analysis of this amplicon. HPV integration into the cellular genome was analyzed by western blot detecting HPV-L1 capsid protein. Presence of HPV was observed in 19 of the 40 pterygia samples. In contrast, healthy conjunctiva samples were negative. To determine virus type, sequence analyses were performed. Interestingly, 11 out of the 19-pterygium samples were identified as HPV-11 type, meanwhile, the remaining 8 pterygium samples were identified as HPV-18. HPV-L1 capsid protein were found only in 3 out of the 10 samples studied. In conclusion, our study identified the presence of HPV DNA exclusively in pterygium samples and described HPV-11 and -18 genotypes. Our results suggest that HPV may be involved in the pathogenesis of pterygium. On the other hand, the expression of the L1-HPV protein suggests viral integration into the cellular genome.
摘要：
翼状胬肉是眼科最常见的病理之一，是良性的，纤维血管组织的过度生长，通常具有机翼状的外观，从角膜上的结膜。它由上皮和高度血管组成，上皮下,疏松的结缔组织。关于翼状胬肉的发病机制有很多争论，已经提出了许多理论，包括遗传不稳定性，细胞增殖，炎症影响，结缔组织退化，血管生成,异常细胞凋亡和病毒感染。目前,人乳头瘤病毒（HPV）参与翼状胬肉的发生是有争议的，据报道，58％的病例中存在HPV，而其他人未能在翼状胬肉中检测到HPV。在这项研究中,我们评估了翼状突和健康结膜样本中HPVDNA的存在和病毒基因型，和病毒整合到细胞基因组中。通过聚合酶链反应分析了40个原发性翼状组织样本和12个健康的结膜样本是否存在HPVDNA，使用MY09/MY11引物检测HPV-L1基因。通过该扩增子的DNA序列分析鉴定病毒基因型。通过蛋白质印迹检测HPV-L1衣壳蛋白分析HPV整合到细胞基因组中。在40个翼状组织样品中的19个中观察到HPV的存在。相比之下,健康结膜样本为阴性.要确定病毒类型，进行序列分析。有趣的是,19例翼状胬肉样本中有11例被鉴定为HPV-11型，同时,其余8例翼状胬肉标本被鉴定为HPV-18.HPV-L1衣壳蛋白仅在所研究的10个样品中的3个中发现。总之,我们的研究确定了翼状胬肉样本中仅存在HPVDNA,并描述了HPV-11和-18基因型.我们的结果表明，HPV可能参与了翼状胬肉的发病机制。另一方面,L1-HPV蛋白的表达提示病毒整合到细胞基因组中.
