PDF(Pubmed)
Abstract：
Onychomycosis is a chronic fungal nail infection caused by several filamentous and yeast-like fungi, such as the genus Candida spp., of great clinical importance. Black yeasts, such as Exophiala dermatitidis, a closely related Candida spp. species, also act as opportunistic pathogens. Fungi infectious diseases are affected by organisms organized in biofilm in onychomycosis, making treatment even more difficult. This study aimed to evaluate the in vitro susceptibility profile to propolis extract and the ability to form a simple and mixed biofilm of two yeasts isolated from the same onychomycosis infection. The yeasts isolated from a patient with onychomycosis were identified as Candida parapsilosis sensu stricto and Exophiala dermatitidis. Both yeasts were able to form simple and mixed (in combination) biofilms. Notably, C. parapsilosis prevailed when presented in combination. The susceptibility profile of propolis extract showed action against E. dermatitidis and C. parapsilosis in planktonic form, but when the yeasts were in mixed biofilm, we only observed action against E. dermatitidis, until total eradication.
摘要：
甲癣是由几种丝状和酵母样真菌引起的慢性真菌指甲感染，如念珠菌属。,具有重要的临床意义。黑色酵母,例如Exophialadermatitidis,密切相关的念珠菌属。物种,也作为机会病原体。真菌感染性疾病受到甲癣生物膜中生物组织的影响，使治疗更加困难。这项研究旨在评估对蜂胶提取物的体外敏感性以及从同一甲癣感染中分离出的两种酵母形成简单混合生物膜的能力。从甲癣患者中分离出的酵母菌被鉴定为严格的近感念珠菌和皮肤外植菌。两种酵母都能够形成简单和混合(组合)的生物膜。值得注意的是,以组合形式存在时，拟态梭菌占上风。蜂胶提取物的敏感性曲线显示了对浮游形式的皮氏芽孢杆菌和近生芽孢杆菌的作用，但是当酵母处于混合生物膜中时，我们只观察到了对E.dermatitidis的作用,直到彻底根除。
