Abstract：
Molecular stable isotope ratios are a novel type of dietary biomarker with high sensitivity and specificity for certain foods. Among these, fatty acid carbon isotope ratios (CIRs) have strong potential but have not been investigated as dietary biomarkers.
We evaluated whether fatty acid CIRs and mass proportions were associated with meat, fish, and sugar-sweetened beverage (SSB) intake.
Thirty-two men [aged 46.2 ± 10.5 y; BMI (kg/m2): 27.2 ± 4.0] underwent a 12-wk inpatient dietary intervention at the National Institute of Diabetes and Digestive and Kidney Diseases in Phoenix, Arizona. Men were randomly assigned to 1 of 8 dietary treatments varying the presence/absence of dietary meat, fish, and SSBs in all combinations. Fatty acid CIRs and mass proportions were measured in fasting blood samples and adipose tissue biopsies that were collected pre- and postintervention. Dietary effects were analyzed using multivariable regression and receiver operating characteristic AUCs were calculated using logistic regression.
CIRs of the several abundant SFAs, MUFAs and arachidonic acid (20:4n-6) in plasma were strongly associated with meat, as were a subset of these fatty acids in RBCs. Effect sizes in plasma ranged from 1.01‰ to 1.93‰ and were similar but attenuated in RBCs. Mass proportions of those fatty acids were not associated with diet. CIRs of plasma dihomo-γ-linolenic acid (20:3n-6) and adipose palmitic acid (16:0) were weakly associated with SSBs. Mass proportions of plasma odd-chain fatty acids were associated with meat, and mass proportions of plasma EPA and DHA (20:5n-3 and 22:6n-3) were associated with fish.
CIRs of plasma and RBC fatty acids show promise as sensitive and specific measures of dietary meat. These provide different information from that provided by fatty acid mass proportions, and are informative where mass proportion is not. This trial is registered at www.
gov as NCT01237093.
We evaluated whether fatty acid CIRs and mass proportions were associated with meat, fish, and sugar-sweetened beverage (SSB) intake.
Thirty-two men [aged 46.2 ± 10.5 y; BMI (kg/m2): 27.2 ± 4.0] underwent a 12-wk inpatient dietary intervention at the National Institute of Diabetes and Digestive and Kidney Diseases in Phoenix, Arizona. Men were randomly assigned to 1 of 8 dietary treatments varying the presence/absence of dietary meat, fish, and SSBs in all combinations. Fatty acid CIRs and mass proportions were measured in fasting blood samples and adipose tissue biopsies that were collected pre- and postintervention. Dietary effects were analyzed using multivariable regression and receiver operating characteristic AUCs were calculated using logistic regression.
CIRs of the several abundant SFAs, MUFAs and arachidonic acid (20:4n-6) in plasma were strongly associated with meat, as were a subset of these fatty acids in RBCs. Effect sizes in plasma ranged from 1.01‰ to 1.93‰ and were similar but attenuated in RBCs. Mass proportions of those fatty acids were not associated with diet. CIRs of plasma dihomo-γ-linolenic acid (20:3n-6) and adipose palmitic acid (16:0) were weakly associated with SSBs. Mass proportions of plasma odd-chain fatty acids were associated with meat, and mass proportions of plasma EPA and DHA (20:5n-3 and 22:6n-3) were associated with fish.
CIRs of plasma and RBC fatty acids show promise as sensitive and specific measures of dietary meat. These provide different information from that provided by fatty acid mass proportions, and are informative where mass proportion is not. This trial is registered at www.
gov as NCT01237093.
摘要：
背景：分子稳定同位素比值是一种新型的饮食生物标志物，对某些食物具有高灵敏度和特异性。其中,脂肪酸碳同位素比值(CIR)具有很强的潜力,但尚未作为膳食生物标志物进行研究.
目的：我们评估了脂肪酸CIRs和质量比例是否与肉类有关，鱼,和含糖饮料（SSB）的摄入量。
方法：32名男性[年龄46.2±10.5岁；BMI（kg/m2）：27.2±4.0]在凤凰城的国立糖尿病，消化和肾脏疾病研究所接受了12周的住院饮食干预，亚利桑那.男性被随机分配到8种饮食治疗中的1种，改变饮食肉的存在/不存在，鱼,和SSB的所有组合。在干预前和干预后收集的空腹血液样本和脂肪组织活检中测量脂肪酸CIRs和质量比例。使用多变量回归分析饮食效果，并使用逻辑回归计算接受者操作特征AUC。
结果：几个丰富的SFA的CIR，血浆中的MUFA和花生四烯酸（20：4n-6）与肉类密切相关，以及红细胞中这些脂肪酸的子集。血浆中的效应大小为1.01‰至1.93‰，在红细胞中相似但减弱。这些脂肪酸的质量比例与饮食无关。血浆二高-γ-亚麻酸（20：3n-6）和脂肪棕榈酸（16：0）的CIRs与SSB弱相关。血浆奇数链脂肪酸的质量比例与肉类有关，血浆EPA和DHA的质量比例（20：5n-3和22：6n-3）与鱼类有关。
结论：血浆和RBC脂肪酸的CIR有望作为膳食肉类的敏感和特异性措施。这些提供了与脂肪酸质量比例不同的信息，并且在质量比例没有的地方提供信息。该试验已在www上注册。
结果：gov为NCT01237093。
目的：我们评估了脂肪酸CIRs和质量比例是否与肉类有关，鱼,和含糖饮料（SSB）的摄入量。
方法：32名男性[年龄46.2±10.5岁；BMI（kg/m2）：27.2±4.0]在凤凰城的国立糖尿病，消化和肾脏疾病研究所接受了12周的住院饮食干预，亚利桑那.男性被随机分配到8种饮食治疗中的1种，改变饮食肉的存在/不存在，鱼,和SSB的所有组合。在干预前和干预后收集的空腹血液样本和脂肪组织活检中测量脂肪酸CIRs和质量比例。使用多变量回归分析饮食效果，并使用逻辑回归计算接受者操作特征AUC。
结果：几个丰富的SFA的CIR，血浆中的MUFA和花生四烯酸（20：4n-6）与肉类密切相关，以及红细胞中这些脂肪酸的子集。血浆中的效应大小为1.01‰至1.93‰，在红细胞中相似但减弱。这些脂肪酸的质量比例与饮食无关。血浆二高-γ-亚麻酸（20：3n-6）和脂肪棕榈酸（16：0）的CIRs与SSB弱相关。血浆奇数链脂肪酸的质量比例与肉类有关，血浆EPA和DHA的质量比例（20：5n-3和22：6n-3）与鱼类有关。
结论：血浆和RBC脂肪酸的CIR有望作为膳食肉类的敏感和特异性措施。这些提供了与脂肪酸质量比例不同的信息，并且在质量比例没有的地方提供信息。该试验已在www上注册。
结果：gov为NCT01237093。
