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Abstract：
UNASSIGNED: Japanese spotted fever (JSF) is a tick-borne bacterial febrile disease caused by Rickettsia japonica characterized by fever, rash, and occasional death. The number of patients in Japan and the Tottori Prefecture has been increasing over the past 20 years. Most cases were found in Eastern Tottori; however, the distribution of patients has expanded to the Central and Western regions. Ticks carried by wild animals may be the cause, but the prevalence of R. japonica in ticks has not yet been analyzed.
UNASSIGNED: Ticks were collected by flagging-dragging from 16 sites in Tottori, Japan. The ticks were morphologically classified and DNA was extracted. The 17-kDa antigen gene was amplified using nested PCR. PCR amplicons from ticks and JSF patients were sequenced and phylogenetically compared.
UNASSIGNED: In total, 177 ticks were collected and identified as Haemahysalis, Ixodes, Amblyomma, and Dermcentor. The Spotted Fever Group Rickettsia (SFGR) was detected in Haemahysalis and Amblyomma spp. using PCR, with positivity rates of 36.8% and 33.3%, respectively. DNA sequencing and phylogenetic analysis revealed that positive ticks harbored R. japonica, P. raoultii, and other Rickettsiae species; however, the patient\'s samples were restricted to R. japonica. Similar to the incidence of JSF, the rate of R. japonica-positive ticks was higher in the Eastern region; however, R. japonica-positive ticks were also detected in the Western region.
UNASSIGNED: R. japonica sequences had been found in ticks collected in Tottori Prefecture. Ticks harboring R. japonica were found in the Eastern and Western parts of Tottori Prefecture and the sequences were identical to the human cases. Only the R. japonica sequence has been detected in patients with spotted fever symptoms, even though ticks were harboring various SFGRs.
UNASSIGNED: Ticks were collected by flagging-dragging from 16 sites in Tottori, Japan. The ticks were morphologically classified and DNA was extracted. The 17-kDa antigen gene was amplified using nested PCR. PCR amplicons from ticks and JSF patients were sequenced and phylogenetically compared.
UNASSIGNED: In total, 177 ticks were collected and identified as Haemahysalis, Ixodes, Amblyomma, and Dermcentor. The Spotted Fever Group Rickettsia (SFGR) was detected in Haemahysalis and Amblyomma spp. using PCR, with positivity rates of 36.8% and 33.3%, respectively. DNA sequencing and phylogenetic analysis revealed that positive ticks harbored R. japonica, P. raoultii, and other Rickettsiae species; however, the patient\'s samples were restricted to R. japonica. Similar to the incidence of JSF, the rate of R. japonica-positive ticks was higher in the Eastern region; however, R. japonica-positive ticks were also detected in the Western region.
UNASSIGNED: R. japonica sequences had been found in ticks collected in Tottori Prefecture. Ticks harboring R. japonica were found in the Eastern and Western parts of Tottori Prefecture and the sequences were identical to the human cases. Only the R. japonica sequence has been detected in patients with spotted fever symptoms, even though ticks were harboring various SFGRs.
摘要：
■日本斑疹热（JSF）是由日本立克次菌引起的以发热为特征的蜱传细菌性发热病，皮疹,偶尔死亡。在过去的20年中，日本和鸟取县的患者人数一直在增加。大多数病例是在东鸟取发现的；然而，患者分布已扩展到中西部地区。野生动物携带的蜱虫可能是原因，但目前还没有分析水稻在蜱中的流行情况。
■通过从鸟取的16个地点进行标记拖动来收集刻度，日本。对蜱进行形态学分类并提取DNA。使用巢式PCR扩增17-kDa抗原基因。对来自蜱和JSF患者的PCR扩增子进行测序并进行系统发育比较。
■总共，收集了177只蜱，并鉴定为Haemahhalis，Ixodes,弱视,和皮肤科。在血囊和弱虫中检测到斑点热组立克次体（SFGR）。使用PCR，阳性率分别为36.8%和33.3%，分别。DNA测序和系统发育分析显示，阳性蜱中含有粳稻，P.raoultii,和其他立克次体物种；然而，患者的样本被限制在日本红。类似于JSF的发生率，东部地区的R.japonica阳性蜱率较高；然而，在西部地区也检测到R.japonica阳性蜱。
■R在鸟取县收集的蜱中发现了粳稻序列。在鸟取县的东部和西部发现了带有R.japonica的ticks，其序列与人类病例相同。仅在有斑点发热症状的患者中检测到R.japonica序列，尽管蜱有各种SFGR。
■通过从鸟取的16个地点进行标记拖动来收集刻度，日本。对蜱进行形态学分类并提取DNA。使用巢式PCR扩增17-kDa抗原基因。对来自蜱和JSF患者的PCR扩增子进行测序并进行系统发育比较。
■总共，收集了177只蜱，并鉴定为Haemahhalis，Ixodes,弱视,和皮肤科。在血囊和弱虫中检测到斑点热组立克次体（SFGR）。使用PCR，阳性率分别为36.8%和33.3%，分别。DNA测序和系统发育分析显示，阳性蜱中含有粳稻，P.raoultii,和其他立克次体物种；然而，患者的样本被限制在日本红。类似于JSF的发生率，东部地区的R.japonica阳性蜱率较高；然而，在西部地区也检测到R.japonica阳性蜱。
■R在鸟取县收集的蜱中发现了粳稻序列。在鸟取县的东部和西部发现了带有R.japonica的ticks，其序列与人类病例相同。仅在有斑点发热症状的患者中检测到R.japonica序列，尽管蜱有各种SFGR。
