PDF(Pubmed)
Abstract：
BACKGROUND: Bariatric procedures are safe and effective treatments for obesity, inducing rapid and sustained loss of excess body weight. Laparoscopic adjustable gastric banding (LAGB) is unique among bariatric interventions in that it is a reversible procedure in which normal gastrointestinal anatomy is maintained. Knowledge regarding how LAGB effects change at the metabolite level is limited.
OBJECTIVE: To delineate the impact of LAGB on fasting and postprandial metabolite responses using targeted metabolomics.
METHODS: Individuals undergoing LAGB at NYU Langone Medical Center were recruited for a prospective cohort study.
METHODS: We prospectively analyzed serum samples from 18 subjects at baseline and 2 months after LAGB under fasting conditions and after a 1-hour mixed meal challenge. Plasma samples were analyzed on a reverse-phase liquid chromatography time-of-flight mass spectrometry metabolomics platform. The main outcome measure was their serum metabolite profile.
RESULTS: We quantitatively detected over 4,000 metabolites and lipids. Metabolite levels were altered in response to surgical and prandial stimuli, and metabolites within the same biochemical class tended to behave similarly in response to either stimulus. Plasma levels of lipid species and ketone bodies were statistically decreased after surgery whereas amino acid levels were affected more by prandial status than surgical condition.
CONCLUSIONS: Changes in lipid species and ketone bodies postoperatively suggest improvements in the rate and efficiency of fatty acid oxidation and glucose handling after LAGB. Further investigation is necessary to understand how these findings relate to surgical response, including long term weight maintenance, and obesity-related comorbidities such as dysglycemia and cardiovascular disease.
OBJECTIVE: To delineate the impact of LAGB on fasting and postprandial metabolite responses using targeted metabolomics.
METHODS: Individuals undergoing LAGB at NYU Langone Medical Center were recruited for a prospective cohort study.
METHODS: We prospectively analyzed serum samples from 18 subjects at baseline and 2 months after LAGB under fasting conditions and after a 1-hour mixed meal challenge. Plasma samples were analyzed on a reverse-phase liquid chromatography time-of-flight mass spectrometry metabolomics platform. The main outcome measure was their serum metabolite profile.
RESULTS: We quantitatively detected over 4,000 metabolites and lipids. Metabolite levels were altered in response to surgical and prandial stimuli, and metabolites within the same biochemical class tended to behave similarly in response to either stimulus. Plasma levels of lipid species and ketone bodies were statistically decreased after surgery whereas amino acid levels were affected more by prandial status than surgical condition.
CONCLUSIONS: Changes in lipid species and ketone bodies postoperatively suggest improvements in the rate and efficiency of fatty acid oxidation and glucose handling after LAGB. Further investigation is necessary to understand how these findings relate to surgical response, including long term weight maintenance, and obesity-related comorbidities such as dysglycemia and cardiovascular disease.
摘要：
背景：减肥手术是治疗肥胖的安全有效方法，诱导快速和持续的过度体重减轻。腹腔镜可调节胃束带术（LAGB）在减肥干预措施中是独特的，因为它是一种可逆的过程，可以维持正常的胃肠道解剖结构。关于LAGB在代谢物水平上如何影响变化的知识是有限的。
目的：使用靶向代谢组学描述LAGB对空腹和餐后代谢产物反应的影响。
方法：招募在NYULangone医学中心接受LAGB的个体进行前瞻性队列研究。
方法：我们前瞻性分析了18名受试者在基线和LAGB后2个月在禁食条件下和1小时混合餐食攻击后的血清样品。在反相液相色谱飞行时间质谱代谢组学平台上分析血浆样品。主要结局指标是他们的血清代谢物谱。
结果：我们定量检测了4,000多种代谢物和脂质。代谢产物水平因手术和餐时刺激而改变,相同生化类别中的代谢物对两种刺激的反应往往相似。手术后血浆脂质和酮体的水平在统计学上降低，而氨基酸水平受餐时状态的影响大于手术状态。
结论：术后脂质种类和酮体的变化提示LAGB后脂肪酸氧化和葡萄糖处理的速率和效率改善。进一步的调查是必要的，以了解这些发现如何与手术反应，包括长期的体重维持，与肥胖相关的合并症，如血糖异常和心血管疾病。
目的：使用靶向代谢组学描述LAGB对空腹和餐后代谢产物反应的影响。
方法：招募在NYULangone医学中心接受LAGB的个体进行前瞻性队列研究。
方法：我们前瞻性分析了18名受试者在基线和LAGB后2个月在禁食条件下和1小时混合餐食攻击后的血清样品。在反相液相色谱飞行时间质谱代谢组学平台上分析血浆样品。主要结局指标是他们的血清代谢物谱。
结果：我们定量检测了4,000多种代谢物和脂质。代谢产物水平因手术和餐时刺激而改变,相同生化类别中的代谢物对两种刺激的反应往往相似。手术后血浆脂质和酮体的水平在统计学上降低，而氨基酸水平受餐时状态的影响大于手术状态。
结论：术后脂质种类和酮体的变化提示LAGB后脂肪酸氧化和葡萄糖处理的速率和效率改善。进一步的调查是必要的，以了解这些发现如何与手术反应，包括长期的体重维持，与肥胖相关的合并症，如血糖异常和心血管疾病。
