PDF(Pubmed)
Abstract：
UNASSIGNED: Hemophore-like proteins sequester heme from host hemoproteins. We aimed to determine whether the host immune system can recognize not only Porphyromonas gingivalis HmuY but also its homologs expressed by other periodontopathogens, and how periodontitis influences the production of respective antibodies.
UNASSIGNED: The reactivity of total bacterial antigens and purified proteins with serum IgG antibodies of 18 individuals with periodontitis and 17 individuals without periodontitis was examined by enzyme-linked immunosorbent assay (ELISA). To compare IgG reactivity between groups with and without periodontitis and within the various dilutions of sera, statistical analysis was performed using the Mann-Whitney U-test and two-way ANOVA test with the post-hoc Bonferroni test.
UNASSIGNED: Individuals with periodontitis produced IgG antibodies reacting more strongly not only with total P. gingivalis antigens (P = 0.0002; 1:400) and P. gingivalis HmuY (P = 0.0016; 1:100) but also with Prevotella intermedia PinA (P = 0.0059; 1:100), and with low efficiency with P. intermedia PinO (P = 0.0021; 1:100). No increase in the reactivity of IgG antibodies with Tannerella forsythia Tfo and P. gingivalis HusA was found in individuals with periodontitis.
UNASSIGNED: Although hemophore-like proteins are structurally related, they are differentially recognized by the host immune system. Our findings point to specific antigens, mainly P. gingivalis HmuY and P. intermedia PinA, whose immunoreactivity could be further investigated to develop markers of periodontitis.
UNASSIGNED: The reactivity of total bacterial antigens and purified proteins with serum IgG antibodies of 18 individuals with periodontitis and 17 individuals without periodontitis was examined by enzyme-linked immunosorbent assay (ELISA). To compare IgG reactivity between groups with and without periodontitis and within the various dilutions of sera, statistical analysis was performed using the Mann-Whitney U-test and two-way ANOVA test with the post-hoc Bonferroni test.
UNASSIGNED: Individuals with periodontitis produced IgG antibodies reacting more strongly not only with total P. gingivalis antigens (P = 0.0002; 1:400) and P. gingivalis HmuY (P = 0.0016; 1:100) but also with Prevotella intermedia PinA (P = 0.0059; 1:100), and with low efficiency with P. intermedia PinO (P = 0.0021; 1:100). No increase in the reactivity of IgG antibodies with Tannerella forsythia Tfo and P. gingivalis HusA was found in individuals with periodontitis.
UNASSIGNED: Although hemophore-like proteins are structurally related, they are differentially recognized by the host immune system. Our findings point to specific antigens, mainly P. gingivalis HmuY and P. intermedia PinA, whose immunoreactivity could be further investigated to develop markers of periodontitis.
摘要：
■类血团蛋白从宿主血液蛋白中螯合血红素。我们旨在确定宿主免疫系统是否不仅可以识别牙龈卟啉单胞菌HmuY，还可以识别其他牙周病原体表达的同源物。以及牙周炎如何影响各自抗体的产生。
■通过酶联免疫吸附测定（ELISA）检查了18例牙周炎个体和17例无牙周炎个体的总细菌抗原和纯化蛋白与血清IgG抗体的反应性。为了比较有和没有牙周炎的组之间的IgG反应性以及在不同稀释度的血清中，统计学分析采用Mann-WhitneyU检验和双因素方差分析检验以及post-hocBonferroni检验.
患有牙周炎的个体产生的IgG抗体不仅与总牙龈卟啉单胞菌抗原（P=0.0002;1:400）和牙龈卟啉单胞菌HmuY（P=0.0016;1:100）反应更强烈，而且与中间型PinA（P=0.0059;1:100）反应更强烈，与P.intermediatePinO（P=0.0021；1：100）的效率较低。在患有牙周炎的个体中没有发现IgG抗体与连翘Tfo和牙龈卟啉单胞菌HusA的反应性增加。
■尽管血团样蛋白在结构上是相关的，它们被宿主免疫系统差异识别。我们的发现指向特定的抗原,主要是牙龈卟啉单胞菌HmuY和中间卟啉单胞菌，其免疫反应性可以进一步研究以开发牙周炎标志物。
■通过酶联免疫吸附测定（ELISA）检查了18例牙周炎个体和17例无牙周炎个体的总细菌抗原和纯化蛋白与血清IgG抗体的反应性。为了比较有和没有牙周炎的组之间的IgG反应性以及在不同稀释度的血清中，统计学分析采用Mann-WhitneyU检验和双因素方差分析检验以及post-hocBonferroni检验.
患有牙周炎的个体产生的IgG抗体不仅与总牙龈卟啉单胞菌抗原（P=0.0002;1:400）和牙龈卟啉单胞菌HmuY（P=0.0016;1:100）反应更强烈，而且与中间型PinA（P=0.0059;1:100）反应更强烈，与P.intermediatePinO（P=0.0021；1：100）的效率较低。在患有牙周炎的个体中没有发现IgG抗体与连翘Tfo和牙龈卟啉单胞菌HusA的反应性增加。
■尽管血团样蛋白在结构上是相关的，它们被宿主免疫系统差异识别。我们的发现指向特定的抗原,主要是牙龈卟啉单胞菌HmuY和中间卟啉单胞菌，其免疫反应性可以进一步研究以开发牙周炎标志物。
