Abstract：
Acid mine drainage (AMD) is known as an important source of environmental pollution with potentially toxic elements. High concentrations of minerals in soil were observed in a pomegranate garden nearby a copper mine, Chaharmahal and Bakhtiari, Iran. In the vicinity of this mine, AMD locally caused distinct chlorosis in pomegranate trees. As expected, potentially toxic concentrations of Cu, Fe, and Zn were accumulated in the leaves of the chlorotic pomegranate trees (YLP), i.e., increased by 69 %, 67 % and 56 %, respectively, compared to the non-chlorotic trees (GLP). Remarkably, also some other elements, including Al (82 %), Na (39 %), Si (87 %), and Sr (69 %) were considerably enhanced in YLP, compared to GLP. On the other hand, the foliar Mn concentration in YLP was strongly decreased, about 62 % lower than that in GLP. The most plausible reasons for chlorosis in YLP are either toxicity of Al, Cu, Fe, Na, and Zn, or a deficiency of Mn. In addition, AMD led to oxidative stress, shown by a high accumulation of H2O2 in YLP, and a strong upregulation of enzymatic and non-enzymatic antioxidants. AMD apparently caused chlorosis, reduced the size of individual leaves, and caused lipid peroxidation. A further analysis of the adverse effect of the responsible AMD component(s) could be helpful to reduce the risk of food chain contamination.
摘要：
酸性矿山排水（AMD）被认为是具有潜在毒性元素的重要环境污染源。在铜矿附近的石榴园中观察到土壤中高浓度的矿物质，Chaharmahal和Bakhtiari,伊朗。在这个矿井附近,AMD在石榴树中局部引起明显的萎黄病。不出所料,铜的潜在毒性浓度，Fe,锌积累在褪绿石榴树（YLP）的叶子中，即，增加了69%,67%和56%，分别,与非褪绿树木（GLP）相比。值得注意的是,还有其他一些元素，包括铝（82%），Na(39%),Si(87%),和Sr(69%)在YLP中显著提高，与GLP相比。另一方面,YLP中的叶面Mn浓度急剧下降，比GLP低约62%。YLP萎黄病最合理的原因是铝的毒性，Cu,Fe,Na,Zn,或者缺乏锰。此外,AMD导致氧化应激，YLP中H2O2的高积累表明，以及酶和非酶抗氧化剂的强烈上调。AMD显然导致萎黄病，减少了单个叶子的大小，并导致脂质过氧化。对负责的AMD组分的不利影响的进一步分析可能有助于降低食物链污染的风险。
