Abstract：
Introduction. Burkholderia thailandensis is a clinically rare opportunistic pathogen in the genus Burkholderia, and the genomic features and virulence characteristics of B. thailandensis strains that cause human infection remain unclear.Gap Statement. B. thailandensis strains with different virulence induce different host innate immune responses in vitro.Aim. This work aimed to understand the sequence diversity, phylogenetic relationship, and virulence of B. thailandensis BPM causing human infection.Methodology. The comparative molecular and genomic analyses, and mouse infection studies were applied to analyse the virulence and genomic features of B. thailandensis BPM originating from China.Results. The whole genome sequence analysis showed that the genomes of BPM and other avirulent B. thailandensis strains were broadly similar, comprising two highly syntenic chromosomes with comparable numbers of coding regions (CDs), protein family distributions, and horizontally acquired genomic islands. By examining species-specific genomic regions, we obtained molecular explanations for previously known differences in virulence and discovered the potential specific virulence-associated genes of BPM, which likely work together to confer the virulence of BPM. Significantly reduced LD50 and survival rates during mouse infection experiments were found in BPM compared to the avirulent B. thailandensis E264 (BtE264).Conclusion. Taken together, the results of this study provide basic information on the genomic features and virulence characteristics of the virulent B. thailandensis strain BPM, which is helpful for understanding its evolution as it relates to pathogenesis and environmental adaptability.
摘要：
Introduction.Thailandensis伯克霍尔德氏菌是伯克霍尔德氏菌属中临床上罕见的机会性病原体，导致人类感染的B.thailandensis菌株的基因组特征和毒力特征尚不清楚。差距声明。具有不同毒力的B.thailandensis菌株在体外诱导不同的宿主先天免疫应答。瞄准.这项工作旨在了解序列的多样性，系统发育关系，以及导致人类感染的B.thailandensisBPM的毒力。方法论。比较分子和基因组分析，和小鼠感染研究用于分析来自中国的B.thailandensisBPM的毒力和基因组特征。结果。全基因组序列分析表明，BPM与其他耐毒株的基因组大致相似，包含两个具有相当数量的编码区（CD）的高度同伦染色体，蛋白质家族分布，和水平获得的基因组岛。通过检查物种特异性基因组区域，我们获得了先前已知的毒力差异的分子解释，并发现了BPM的潜在特定毒力相关基因，它们可能一起工作以赋予BPM的毒力。与无毒B.thailandensisE264（BtE264）相比，在BPM中发现了小鼠感染实验期间LD50和存活率的显着降低。结论。一起来看,这项研究的结果提供了有关强毒株BPM的基因组特征和毒力特征的基本信息。这有助于理解它的进化，因为它与发病机理和环境适应性有关。
