PDF(Pubmed)
Abstract：
UNASSIGNED: Intestinal leakage commonly occurs in severe dengue infection with zonulin as a biomarker. The aim of this study was to determine the effects of NS1 on liver weight, zonulin expression and serum zonulin levels.
UNASSIGNED: This laboratory experiment used 18 ddY mice, which were randomly divided into control (C), PBS (T1), and PBS + NS1 (T2) groups. Mice in the T1 and T2 groups were intravenously injected with 500 μl PBS only and 50 μg NS1 respectively. Mice blood samples were collected before and after three-day treatment for measurement of zonulin level. The fresh liver was weighted directly and were then used for immunostaining.
UNASSIGNED: The C group had lower wet liver weight compared to the T groups (p=0.001). Increased expression of liver zonulin was found in the T2 group, significant different from the C (p=0.014) and T1 groups (p=0.020). After treatment, serum zonulin levels in the T1 group was higher than that of the T1 group before treatment (p=0.035) but not in control (p=0.753) and T2 groups (p=0.869).
UNASSIGNED: Administration of 50 μg NS 1 increases wet liver weight and zonulin expression in hepatocytes, but did not increase serum zonulin levels in ddY mice.
UNASSIGNED: This laboratory experiment used 18 ddY mice, which were randomly divided into control (C), PBS (T1), and PBS + NS1 (T2) groups. Mice in the T1 and T2 groups were intravenously injected with 500 μl PBS only and 50 μg NS1 respectively. Mice blood samples were collected before and after three-day treatment for measurement of zonulin level. The fresh liver was weighted directly and were then used for immunostaining.
UNASSIGNED: The C group had lower wet liver weight compared to the T groups (p=0.001). Increased expression of liver zonulin was found in the T2 group, significant different from the C (p=0.014) and T1 groups (p=0.020). After treatment, serum zonulin levels in the T1 group was higher than that of the T1 group before treatment (p=0.035) but not in control (p=0.753) and T2 groups (p=0.869).
UNASSIGNED: Administration of 50 μg NS 1 increases wet liver weight and zonulin expression in hepatocytes, but did not increase serum zonulin levels in ddY mice.
摘要：
■肠漏通常发生在以zonulin作为生物标志物的严重登革热感染中。这项研究的目的是确定NS1对肝脏重量的影响，zonulin表达和血清zonulin水平。
■这项实验室实验使用了18只ddY小鼠，随机分为对照(C)，PBS（T1），和PBS+NS1(T2)组。T1和T2组中的小鼠分别静脉内注射500μlPBS和50μgNS1。在三天处理之前和之后收集小鼠血液样品以测量连蛋白水平。新鲜肝脏直接称重,然后用于免疫染色。
■与T组相比，C组肝湿重较低（p=0.001）。在T2组中发现肝脏zonulin的表达增加，与C组(p=0.014)和T1组(p=0.020)有显著差异。治疗后,T1组的血清zonulin水平高于治疗前的T1组（p=0.035），但对照组（p=0.753）和T2组（p=0.869）没有。
■施用50μgNS1可增加肝湿重和肝细胞中连蛋白表达，但没有增加ddY小鼠的血清zonulin水平。
■这项实验室实验使用了18只ddY小鼠，随机分为对照(C)，PBS（T1），和PBS+NS1(T2)组。T1和T2组中的小鼠分别静脉内注射500μlPBS和50μgNS1。在三天处理之前和之后收集小鼠血液样品以测量连蛋白水平。新鲜肝脏直接称重,然后用于免疫染色。
■与T组相比，C组肝湿重较低（p=0.001）。在T2组中发现肝脏zonulin的表达增加，与C组(p=0.014)和T1组(p=0.020)有显著差异。治疗后,T1组的血清zonulin水平高于治疗前的T1组（p=0.035），但对照组（p=0.753）和T2组（p=0.869）没有。
■施用50μgNS1可增加肝湿重和肝细胞中连蛋白表达，但没有增加ddY小鼠的血清zonulin水平。
