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Abstract：
The prevalence of pediatric obesity is rising rapidly worldwide, and \"omic\" approaches are helpful in investigating the molecular pathophysiology of obesity. This work aims to identify transcriptional differences in the subcutaneous adipose tissue (scAT) of children with overweight (OW), obesity (OB), or severe obesity (SV) compared with those of normal weight (NW). Periumbilical scAT biopsies were collected from 20 male children aged 1-12 years. The children were stratified into the following four groups according to their BMI z-scores: SV, OB, OW, and NW. scAT RNA-Seq analyses were performed, and a differential expression analysis was conducted using the DESeq2 R package. A pathways analysis was performed to gain biological insights into gene expression. Our data highlight the significant deregulation in both coding and non-coding transcripts in the SV group when compared with the NW, OW, and OB groups. A KEGG pathway analysis showed that coding transcripts were mainly involved in lipid metabolism. A GSEA analysis revealed the upregulation of lipid degradation and metabolism in SV vs. OB and SV vs. OW. Bioenergetic processes and the catabolism of branched-chain amino acids were upregulated in SV compared with OB, OW, and NW. In conclusion, we report for the first time that a significant transcriptional deregulation occurs in the periumbilical scAT of children with severe obesity compared with those of normal weight or those with overweight or mild obesity.
摘要：
儿童肥胖的患病率在全球范围内迅速上升,和“组学”方法有助于研究肥胖的分子病理生理学。这项工作旨在确定超重（OW）儿童皮下脂肪组织（SCAT）的转录差异，肥胖（OB），或重度肥胖（SV）与正常体重（NW）相比。收集了20名1-12岁的男性儿童的脐周scAT活检。根据BMIz评分将儿童分为以下四组：SV，OB,OW,和NW。进行了SCATRNA-Seq分析，并使用DESeq2R包进行差异表达分析。进行途径分析以获得对基因表达的生物学见解。我们的数据突出表明，与NW相比，SV组中编码和非编码转录物的显着失调，OW,和OB组。KEGG通路分析表明，编码转录本主要参与脂质代谢。GSEA分析显示SV与SV中脂质降解和代谢的上调OB和SVvs.OW.与OB相比，SV中的生物能量过程和支链氨基酸的分解代谢上调，OW,和NW。总之,我们首次报道,与体重正常或超重或轻度肥胖儿童相比,重度肥胖儿童脐周scAT出现显著的转录失调.
