HGFs were obtained from subcultures of biopsies from clinically healthy gingival tissues of patients undergoing oral surgeries. Cell cytotoxicity assay was used to determine the effect of different concentrations of MF on viability of HGFs. HGFs were then incubated and treated with different concentrations of MF and Porphyromonas gingivais (Pg) LPS. MMP-1, MMP-2, MMP-8, MMP-9, IL-1β, and IL-8 expression analysis was performed using xMAP technology (Luminex 200, Luminex, Austin, TX, USA). Student\'s t-test for a single sample was used to compare the mean values of the study groups with the control value. A p-value of <0.05 and 95% confidence intervals were used to report the statistical significance and precision of mean values.
Concentrations of 0.5, 1- and 2-mM MF had a minimal non-significant cytotoxic effect on the HGFs and caused statistically significant reduction of MMP-1, MMP-2, MMP-8 and IL-8 expressed by the LPS-stimulated HGFs.
The results of the present study confirm that MF suppresses MMP-1, MMP-2, MMP-8 and IL-8 in LPS-stimulated HGFs suggesting an anti-inflammatory effect of MF and potential adjunct therapeutic role in the treatment of periodontal diseases.
方法:HGFs是从口腔手术患者临床健康牙龈组织活检的传代培养中获得的。细胞毒性测定用于确定不同浓度的MF对HGF活力的影响。然后孵育HGF并用不同浓度的MF和牙龈卟啉单胞菌(Pg)LPS处理。MMP-1,MMP-2,MMP-8,MMP-9,IL-1β,和IL-8表达分析使用xMAP技术(Luminex200,Luminex,奥斯汀,TX,美国)。使用单个样品的Studentt检验将研究组的平均值与对照值进行比较。使用<0.05和95%置信区间的p值报告平均值的统计显著性和精确度。
结果:浓度的0.5、1-和2-mMMF对HGF具有最小的不显著的细胞毒性作用,并引起由LPS刺激的HGF表达的MMP-1、MMP-2、MMP-8和IL-8的统计学显著降低。
结论:本研究的结果证实,MF抑制LPS刺激的HGFs中的MMP-1,MMP-2,MMP-8和IL-8,表明MF的抗炎作用以及在治疗牙周病中的潜在辅助治疗作用。