PDF(Pubmed)
Abstract：
Transient receptor potential vanilloid 1 (TRPV1) is a non-selective cation channel with polymodal sensory function. TRPV1 links to fever, while, according to previous studies on TRPV1 knock-out (KO) mice, the role of the channel in the generation of febrile seizure is debated. In the hippocampal formation, functional TRPV1 channels are expressed by Cajal-Retzius cells, which have a role in guidance of migrating neurons during development. Despite the developmental aspects of febrile seizure as well as of Cajal-Retzius cells, no information is available about the hippocampal development in TRPV1 KO mouse. Therefore, in the present work postnatal development of the hippocampal formation was studied in TRPV1 KO mice. Several morphological characteristics including neuronal positioning and maturation, synaptogenesis and myelination were examined with light microscopy following immunohistochemical detection of protein markers of various neurons, synapses, and myelination. Regarding the cytoarchitectonics, neuronal migration, morphological, and neurochemical maturation, no substantial difference could be detected between TRPV1 KO and wild-type control mice. Our data indicate that synapse formation and myelination occur similarly in TRPV1 KO and in control animals. We have found slightly, but not significantly larger numbers of persisting Cajal-Retzius cells in the KO mice than in controls. Our result strengthens previous suggestion concerning the role of TRPV1 channel in the postnatal apoptotic cell death of Cajal-Retzius cells. However, the fact that the hippocampus of KO mice lacks major developmental abnormalities supports the use of TRPV1 KO in various animal models of diseases and pathological conditions.
摘要：
瞬时受体电位香草酸1（TRPV1）是一种具有多模态感觉功能的非选择性阳离子通道。TRPV1与发烧有关，while,根据以前对TRPV1敲除（KO）小鼠的研究，该通道在高热惊厥产生中的作用存在争议。在海马结构中，功能性TRPV1通道由Cajal-Retzius细胞表达，在发育过程中对神经元迁移有指导作用。尽管高热性癫痫发作以及Cajal-Retzius细胞的发育方面，没有关于TRPV1KO小鼠海马发育的信息。因此,在目前的工作中，在TRPV1KO小鼠中研究了海马结构的出生后发育。几种形态特征，包括神经元定位和成熟，在免疫组织化学检测各种神经元的蛋白质标记后，用光学显微镜检查突触发生和髓鞘形成，突触，和髓鞘形成。关于细胞结构学，神经元迁移，形态学,和神经化学成熟，在TRPV1KO和野生型对照小鼠之间没有检测到实质性差异。我们的数据表明，在TRPV1KO和对照动物中，突触形成和髓鞘形成的发生相似。我们发现，但KO小鼠中持续存在的Cajal-Retzius细胞的数量并不明显多于对照组。我们的结果加强了先前关于TRPV1通道在Cajal-Retzius细胞出生后凋亡细胞死亡中的作用的建议。然而,KO小鼠的海马体缺乏主要发育异常的事实支持TRPV1KO在各种疾病和病理状况的动物模型中的使用。
