Abstract：
OBJECTIVE: This study evaluated the effect of cervical margin relocation (CMR) with two different materials and contamination with hemostatic agents on the margin adaptation and microleakage of ceramic restorations.
METHODS: Mesial-occlusal-distal cavities were prepared in 60 human first molars and distributed to 3 groups (n = 20) according to the margin relocation procedure. The groups were: group F; flowable composite applied in two 2 mm increments, group B; bulk-fill flowable composite applied as a bulk increment of 4 mm thickness and group C (control); no CMR was done. Each group was subdivided into two subgroups (subgroup N; no hemostatic agent applied and subgroup H; hemostatic agent was applied). In all groups, ceramic inlays were prepared and cemented. The samples were subjected to thermocycling (10,000 cycles). The adaptation of the cervical margin was evaluated with scanning electron microscopy (200×). Samples were then assessed for microleakage analysis with the dye penetration method. Marginal adaptation data were normally distributed and analyzed using two-way ANOVA followed by Tukey\'s post hoc test. Ordinal microleakage score data were analyzed using cumulative link models followed by the analysis of deviance using Wald chi-square tests.
RESULTS: Both CMR and contamination with a hemostatic agent had significant effects on the margin adaptation of the cervical margin. Group C showed the highest adaptation with no significant difference from group F. The lowest adaptation was revealed in group B with a significant difference from group C. Subgroup N (in all groups) showed a statistically higher adaptation than subgroup H. Regarding microleakage assessment, CMR had no significant effect but hemostatic agent application showed a significantly higher microleakage score for all groups.
CONCLUSIONS: Both the CMR procedure and contamination with AlCl3 hemostatic agent had a negative effect on marginal adaptation. For microleakage assessment, only contamination with hemostatic agent negatively affected the microleakage with no effect on the margin relocation procedure.
METHODS: Mesial-occlusal-distal cavities were prepared in 60 human first molars and distributed to 3 groups (n = 20) according to the margin relocation procedure. The groups were: group F; flowable composite applied in two 2 mm increments, group B; bulk-fill flowable composite applied as a bulk increment of 4 mm thickness and group C (control); no CMR was done. Each group was subdivided into two subgroups (subgroup N; no hemostatic agent applied and subgroup H; hemostatic agent was applied). In all groups, ceramic inlays were prepared and cemented. The samples were subjected to thermocycling (10,000 cycles). The adaptation of the cervical margin was evaluated with scanning electron microscopy (200×). Samples were then assessed for microleakage analysis with the dye penetration method. Marginal adaptation data were normally distributed and analyzed using two-way ANOVA followed by Tukey\'s post hoc test. Ordinal microleakage score data were analyzed using cumulative link models followed by the analysis of deviance using Wald chi-square tests.
RESULTS: Both CMR and contamination with a hemostatic agent had significant effects on the margin adaptation of the cervical margin. Group C showed the highest adaptation with no significant difference from group F. The lowest adaptation was revealed in group B with a significant difference from group C. Subgroup N (in all groups) showed a statistically higher adaptation than subgroup H. Regarding microleakage assessment, CMR had no significant effect but hemostatic agent application showed a significantly higher microleakage score for all groups.
CONCLUSIONS: Both the CMR procedure and contamination with AlCl3 hemostatic agent had a negative effect on marginal adaptation. For microleakage assessment, only contamination with hemostatic agent negatively affected the microleakage with no effect on the margin relocation procedure.
摘要：
目的：本研究评估了两种不同材料的宫颈边缘重新定位和止血剂污染对陶瓷修复体边缘适应和微渗漏的影响。
方法：在60个人类第一磨牙中制备内侧咬合远端腔，并根据边缘重新定位程序分配到3组（n=20）。这些组是：F组；以两个2mm的增量应用可流动复合材料，B组；散装填充可流动复合材料作为4毫米厚度的散装增量和C组（对照）；没有进行宫颈边缘移位。每组被细分为2个亚组(亚组N;不应用止血剂和亚组H;应用止血剂)。在所有组中,陶瓷嵌体准备和胶结。对样品进行热循环(10,000次循环)。用扫描电子显微镜（200×）评估子宫颈边缘的适应。然后用染料渗透法评估样品的微渗漏分析。边际适应数据呈正态分布，并使用双向方差分析进行分析，然后进行Tukey的事后检验。使用累积链接模型分析有序微泄漏评分数据，然后使用Wald卡方检验分析偏差。
结果：宫颈切缘移位和止血剂污染对宫颈切缘适应均有显著影响。C组的适应程度最高，与F组无显著差异。B组的适应程度最低，与C组有显著差异。宫颈切缘定位无显著影响,但止血剂应用显示所有组的微渗漏评分均显著较高.
结论：宫颈边缘重新定位手术和ALCL3止血剂污染对边缘适应都有负面影响。对于微泄漏评估，只有止血剂的污染会对微渗漏产生负面影响，而对边缘重新定位程序没有影响。本文受版权保护。保留所有权利。
方法：在60个人类第一磨牙中制备内侧咬合远端腔，并根据边缘重新定位程序分配到3组（n=20）。这些组是：F组；以两个2mm的增量应用可流动复合材料，B组；散装填充可流动复合材料作为4毫米厚度的散装增量和C组（对照）；没有进行宫颈边缘移位。每组被细分为2个亚组(亚组N;不应用止血剂和亚组H;应用止血剂)。在所有组中,陶瓷嵌体准备和胶结。对样品进行热循环(10,000次循环)。用扫描电子显微镜（200×）评估子宫颈边缘的适应。然后用染料渗透法评估样品的微渗漏分析。边际适应数据呈正态分布，并使用双向方差分析进行分析，然后进行Tukey的事后检验。使用累积链接模型分析有序微泄漏评分数据，然后使用Wald卡方检验分析偏差。
结果：宫颈切缘移位和止血剂污染对宫颈切缘适应均有显著影响。C组的适应程度最高，与F组无显著差异。B组的适应程度最低，与C组有显著差异。宫颈切缘定位无显著影响,但止血剂应用显示所有组的微渗漏评分均显著较高.
结论：宫颈边缘重新定位手术和ALCL3止血剂污染对边缘适应都有负面影响。对于微泄漏评估，只有止血剂的污染会对微渗漏产生负面影响，而对边缘重新定位程序没有影响。本文受版权保护。保留所有权利。
