PDF(Pubmed)
Abstract：
Feline panleukopenia virus (FPV) is the causative agent of hemorrhagic gastroenteritis in feline animals. FPV has been evolving over time, and there have been several different strains of the virus identified. Some of these strains may be more virulent or more resistant to current vaccines than others, which highlights the importance of ongoing research and monitoring of FPV evolution. For FPV genetic evolution analysis, many studies focus on the main capsid protein (VP2), but limited information is available on the nonstructural gene NS1 and structural gene VP1. In the present study, we firstly isolated two novel FPV strains circulating in Shanghai, China, and performed full-length genome sequencing for the desired strains. Subsequently, we focused on analyzing the NS1, VP1 gene, and the encoding protein, and conducted a comparative analysis among the worldwide circulating FPV and Canine parvovirus Type 2 (CPV-2) strains, which included the strains isolated in this study. We found that the 2 structural viral proteins, VP1 and VP2, are splice variants, and VP1 has a 143 amino-acid-long N-terminal compared to VP2. Furthermore, phylogenetic analysis showed that divergent evolution between FPV and CPV-2 virus strains were clustered mostly by country and year of detection. In addition, much more continuous antigenic type changes happened in the process of CPV-2 circulating and evolution compared to FPV. These results stress the importance of the continuous study of viral evolution and provide a comprehensive perspective of the association between viral epidemiology and genetic evolution.
摘要：
猫泛白细胞减少症病毒(FPV)是猫科动物出血性胃肠炎的病原体。FPV一直在不断发展，已经鉴定出几种不同的病毒株。这些菌株中的一些可能比其他菌株更具毒性或对当前疫苗更具抗性，这突出了正在进行的研究和监测FPV演变的重要性。对于FPV遗传进化分析，许多研究集中在主要衣壳蛋白(VP2)上，但是关于非结构基因NS1和结构基因VP1的信息有限。在本研究中,我们首先分离出了两个在上海流行的新型FPV菌株，中国,并对所需菌株进行全长基因组测序。随后，我们专注于分析NS1，VP1基因，和编码蛋白质，并在全球流行的FPV和犬细小病毒2型（CPV-2）株之间进行了比较分析，其中包括本研究中分离出的菌株。我们发现两种结构病毒蛋白，VP1和VP2是剪接变体，与VP2相比,VP1具有143个氨基酸长的N-末端。此外，系统发育分析表明，FPV和CPV-2病毒株之间的进化差异主要集中在国家和检测年份。此外,与FPV相比，CPV-2的循环和进化过程中发生了更多的连续抗原类型变化。这些结果强调了持续研究病毒进化的重要性，并提供了病毒流行病学与遗传进化之间关联的全面观点。
