PDF(Pubmed)
Abstract：
Rosacea is a chronic inflammatory skin disease whose late manifestations have not yet been clearly reported in animal models. The objective of this study is to describe the skin lesions and major histopathological changes in a rosacea-like phenotype in mice induced by prolonged LL-37 administration and furthermore, to assess the potential of long-term LL-37 administration in inducing irreversible rosacea-like skin lesion models. Balb/c mice were continuously injected intradermally with LL-37 every 12 h to induce a rosacea-like phenotype. After LL-37 injections were administered for 20 consecutive days, the area of rosacea-like lesions gradually expanded in the first 13 days, then entered a stable phase. Haematoxylin and eosin (H&E) and Van Gieson\'s staining showed a high degree of inflammatory cell aggregation, thickening of the epidermis and dermis, and collagen deposition in large quantities. The results of immunofluorescence staining and Western blotting showed that the expression of α-SMA, TNF-α, vimentin, and COL1 in the skin of mice was significantly upregulated. Short-term LL-37 administration induced rosacea-like lesions that only featured the aggregation of inflammatory factors and thickening of the epidermis, whereas no collagen hyperplasia was observed, and a full recovery was noticed. However, rosacea-like skin lesions induced by long-term LL-37 administration did not completely recover. Our study compares rosacea-like lesions induced by short-term versus long-term LL-37 administration, and the results suggest that irreversible rosacea-like lesions can be induced by long-term LL-37 administration.
摘要：
酒渣鼻是一种慢性炎症性皮肤病，其晚期表现在动物模型中尚未明确报道。这项研究的目的是描述皮肤损伤和主要的组织病理学的变化，在小鼠的红斑痤疮样表型的长期施用，评估长期施用LL-37诱导不可逆酒渣鼻样皮肤损伤模型的潜力。Balb/c小鼠每12小时连续皮内注射LL-37以诱导红斑痤疮样表型。LL-37连续注射20天后，酒渣鼻样病变的面积在最初的13天逐渐扩大，然后进入稳定阶段。苏木素和伊红（H&E）和VanGieson染色显示高度的炎症细胞聚集，表皮和真皮增厚，和大量的胶原蛋白沉积。免疫荧光染色和Westernblotting结果显示,α-SMA的表达,TNF-α,波形蛋白,小鼠皮肤中COL1明显上调。短期LL-37给药诱导红斑痤疮样病变，仅表现为炎症因子聚集和表皮增厚，而没有观察到胶原蛋白增生，并注意到完全康复。然而,长期给予LL-37诱导的酒渣鼻样皮肤损伤并未完全恢复。我们的研究比较了短期与长期LL-37给药诱导的酒渣鼻样病变，结果表明，长期施用LL-37可以诱发不可逆的酒渣鼻样病变。
