PDF(Pubmed)
Abstract：
UNASSIGNED: Recently, for various reasons, the need for non-invasive treatment for localized fat has emerged. This study confirmed whether Magnolia officinalis (MO) pharmacopuncture reduces localized fat by promoting lipolysis and inhibiting adipogenesis.
UNASSIGNED: The network was built using genes related to the active compound of MO and the mode of action of MO was predicted by the functional enrichment analysis. Based on the result from network analysis, 100 µL of 2 mg/mL MO pharmacopuncture was injected into the inguinal fat pad for 6 weeks in obese C57BL/6J mice. Normal saline was injected into the right-side inguinal fat pad as a self-control.
UNASSIGNED: It was expected that the \'AMP-activated protein kinase (AMPK) signaling pathway\' would be affected by the MO Network. MO pharmacopuncture reduced the weight and size of inguinal fat in HFD-induced obese mice. The phosphorylation of AMPK along with the increases of lipases was significantly increased by MO injection. Also, the expression levels of fatty acid synthesize-related mediators were suppressed by MO injection.
UNASSIGNED: Our results demonstrated that MO pharmacopuncture promoted the expression of AMPK, which has beneficial effects on activation of lipolysis and inhibition of lipogenesis. Pharmacopuncture of MO can be a non-surgical alternative therapy in the treatment of local fat tissue.
UNASSIGNED: The network was built using genes related to the active compound of MO and the mode of action of MO was predicted by the functional enrichment analysis. Based on the result from network analysis, 100 µL of 2 mg/mL MO pharmacopuncture was injected into the inguinal fat pad for 6 weeks in obese C57BL/6J mice. Normal saline was injected into the right-side inguinal fat pad as a self-control.
UNASSIGNED: It was expected that the \'AMP-activated protein kinase (AMPK) signaling pathway\' would be affected by the MO Network. MO pharmacopuncture reduced the weight and size of inguinal fat in HFD-induced obese mice. The phosphorylation of AMPK along with the increases of lipases was significantly increased by MO injection. Also, the expression levels of fatty acid synthesize-related mediators were suppressed by MO injection.
UNASSIGNED: Our results demonstrated that MO pharmacopuncture promoted the expression of AMPK, which has beneficial effects on activation of lipolysis and inhibition of lipogenesis. Pharmacopuncture of MO can be a non-surgical alternative therapy in the treatment of local fat tissue.
摘要：
■最近，出于各种原因,对局部脂肪的非侵入性治疗的需求已经出现。这项研究证实了厚朴（MO）药物穿刺是否通过促进脂解和抑制脂肪生成来减少局部脂肪。
■使用与MO的活性化合物相关的基因构建网络，并通过功能富集分析预测MO的作用方式。根据网络分析的结果，在肥胖的C57BL/6J小鼠中，将100μL的2mg/mLMO药物穿刺注射到腹股沟脂肪垫中，持续6周。将生理盐水注入右侧腹股沟脂肪垫作为自我对照。
■预计“AMP激活的蛋白激酶（AMPK）信号通路”将受到MO网络的影响。MO药物穿刺减少了HFD诱导的肥胖小鼠腹股沟脂肪的重量和大小。通过MO注射，AMPK的磷酸化随着脂肪酶的增加而显着增加。此外，通过MO注射抑制脂肪酸合成相关介质的表达水平。
■我们的结果表明，MO药物穿刺促进了AMPK的表达，这对脂肪分解的激活和脂肪生成的抑制具有有益的作用。MO的药物穿刺可以是治疗局部脂肪组织的非手术替代疗法。
■使用与MO的活性化合物相关的基因构建网络，并通过功能富集分析预测MO的作用方式。根据网络分析的结果，在肥胖的C57BL/6J小鼠中，将100μL的2mg/mLMO药物穿刺注射到腹股沟脂肪垫中，持续6周。将生理盐水注入右侧腹股沟脂肪垫作为自我对照。
■预计“AMP激活的蛋白激酶（AMPK）信号通路”将受到MO网络的影响。MO药物穿刺减少了HFD诱导的肥胖小鼠腹股沟脂肪的重量和大小。通过MO注射，AMPK的磷酸化随着脂肪酶的增加而显着增加。此外，通过MO注射抑制脂肪酸合成相关介质的表达水平。
■我们的结果表明，MO药物穿刺促进了AMPK的表达，这对脂肪分解的激活和脂肪生成的抑制具有有益的作用。MO的药物穿刺可以是治疗局部脂肪组织的非手术替代疗法。
