PDF(Pubmed)
Abstract：
Background: Herbs originating from the Aconitum L. (Ranunculaceae), such as Aconitum carmichaelii Debeaux. (Wutou), Aconitum pendulum Busch. (Tiebangchui), and Aconitum kusnezoffii Reichb. (Caowu), etc. are highly valued for their medicinal properties. The roots and tubers of these herbs are commonly used to treat an array of ailments, including joint pain and tumors. The alkaloids present in them are the primary active components, with aconitine being the most notable. Aconitine has gained attention for its exceptional anti-inflammatory and analgesic properties, as well as its potential as an anti-tumor and cardiotonic agent. However, the exact process through which aconitine hinders the growth of cancerous cells and triggers their programmed cell death remains unclear. Therefore, we have undertaken a comprehensive systematic review and meta-analysis of the current research on the potential antitumor properties of aconitine. Methods: We conducted a thorough search of relevant preclinical studies in databases including PubMed, Web of Science, VIP, WanFang Data, CNKI, Embase, Cochrane Library, and National Center for Biotechnology Information (NCBI). The search was conducted up until 15 September 2022, and the data were statistically analyzed using RevMan 5.4 software. The number of tumor cell value-added, tumor cell apoptosis rate, thymus index (TI), and Bcl-2 gene expression level were the main indicators to be analyzed. Results: After applying the final inclusion criteria, a total of thirty-seven studies, comprising both in vivo and in vitro research were analyzed. The results showed that treatment with aconitine led to a significant reduction in tumor cell proliferation, a noteworthy increase in the rate of apoptosis among tumor cells, a decrease in the thymus index, and a reduction in the expression level of Bcl-2. These results suggested that aconitine could inhibit the proliferation, invasion, and migration abilities of tumor cells by regulating Bcl-2 etc., thereby enhancing the anti-tumor effects. Conclusion: In summary, our present study demonstrated that aconitine effectively reduced tumor size and volume, indicating a strong anti-tumor effect. Additionally, aconitine could increase the expression levels of caspase-3, Bax and other targets. Mechanistically, it may regulate the expression levels of Bax and Bcl-2 through the NF-κB signaling pathway, ultimately inhibiting tumor cell proliferation through autophagy.
摘要：
背景：源自乌头属的草药。如乌头carmichaeliiDebeaux。(乌头),乌头钟摆布希。(铁邦楚)，和乌头草。（Caowu），等。因其药用特性而受到高度重视。这些草药的根和块茎通常用于治疗一系列疾病，包括关节痛和肿瘤.其中存在的生物碱是主要的活性成分，最著名的是乌头碱。乌头碱因其特殊的抗炎和镇痛特性而受到关注，以及它作为抗肿瘤和强心剂的潜力。然而,乌头碱阻碍癌细胞生长并引发其程序性细胞死亡的确切过程仍不清楚。因此,我们对乌头碱的潜在抗肿瘤特性进行了全面的系统评价和荟萃分析。方法：我们在包括PubMed在内的数据库中对相关的临床前研究进行了彻底的搜索，WebofScience,VIP,万方数据,CNKI,Embase,科克伦图书馆,国家生物技术信息中心（NCBI）。搜索进行至2022年9月15日，并使用RevMan5.4软件对数据进行统计分析。肿瘤细胞的数量增值，肿瘤细胞凋亡率，胸腺指数（TI），和Bcl-2基因表达水平是需要分析的主要指标。结果：应用最终纳入标准后，共进行了37项研究,包括体内和体外研究进行了分析。结果表明，乌头碱治疗导致肿瘤细胞增殖显著减少，肿瘤细胞凋亡率显著增加，胸腺指数下降，以及Bcl-2表达水平的降低。这些结果表明乌头碱可以抑制细胞增殖，入侵,通过调控Bcl-2等对肿瘤细胞迁移能力的影响。,从而增强抗肿瘤作用。结论：总之，我们目前的研究表明，乌头碱有效地减少肿瘤的大小和体积，表明有很强的抗肿瘤作用。此外,乌头碱可以提高caspase-3、Bax等靶基因的表达水平。机械上,可能通过NF-κB信号通路调节Bax和Bcl-2的表达,最终通过自噬抑制肿瘤细胞增殖。
