PDF(Pubmed)
Abstract：
The CpG oligodeoxynucleotides (CpG ODNs) reportedly possess the capacity to strengthen immunity in mammals. This experiment was conducted to evaluate the impact of dietary supplementation with 17 types of CpG ODNs on intestinal microbiota diversity, antioxidant capacity, and immune-related gene expression profiles of the shrimp Litopenaeus vannamei. Diets including 50 mg kg-1 CpG ODNs wrapped in egg whites were prepared and divided into 17 different groups, with 2 control groups (normal feed and feed with egg whites). These CpG ODNs supplemented diets and the control diets were fed to L. vannamei (5.15 ± 0.54 g) three times daily at 5%-8% shrimp body weight for three weeks. The results of consecutive detection of intestinal microbiota by 16S rDNA sequencing indicated that 11 of the 17 types of CpG ODNs significantly enhanced intestinal microbiota diversity, increased the populations of several probiotic bacteria, and activated possible mechanisms relevant to diseases. The immune-related genes expression and antioxidant capacity in hepatopancreas further demonstrated that the 11 types of CpG ODNs effectively improved the innate immunity of shrimp. Additionally, histology results showed that the CpG ODNs in the experiment did not damage the tissue structure of hepatopancreas. The results suggest that CpG ODNs could be used as a trace supplement to improve the intestinal health and immunity of shrimp.
摘要：
据报道,CpG寡脱氧核苷酸(CpGODN)具有增强哺乳动物免疫力的能力。本实验旨在评估17种CpGODN对肠道菌群多样性的影响。抗氧化能力，凡纳滨对虾免疫相关基因表达谱。准备包括包裹在蛋白中的50mgkg-1CpGODN的饮食，并分为17个不同的组，2个对照组（正常饲料和蛋清饲料）。将这些补充CpGODN的饮食和对照饮食以5％-8％的虾体重每天三次饲喂南美白对虾乳杆菌（5.15±0.54g），持续三周。16SrDNA测序连续检测肠道菌群的结果表明,17种CpGODNs中有11种显著增强了肠道菌群的多样性,增加了几种益生菌的数量，并激活与疾病相关的可能机制。肝胰腺中免疫相关基因的表达和抗氧化能力进一步证明了11种CpGODN有效地提高了对虾的先天免疫。此外,组织学结果显示,本实验中CpGODNs未破坏肝胰腺组织结构。结果表明，CpGODN可作为微量补充剂，改善对虾的肠道健康和免疫力。
