PDF(Pubmed)
Abstract：
The tumor microenvironment (TME) is constituted by a great diversity of highly dynamic cell populations, each of which contributes ligands, receptors, soluble proteins, mRNAs, and miRNAs, in order to regulate cellular activities within the TME and even promote processes such as angiogenesis or metastasis. Intravasated platelets (PLT) undergo changes in the TME that convert them into tumor-educated platelets (TEP), which supports the development of cancer, angiogenesis, and metastasis through the degranulation and release of biomolecules. Several authors have reported that the deregulation of PF4, VEGF, PDGF, ANG-1, WASF3, LAPTM4B, TPM3, and TAC1 genes participates in breast cancer progression, angiogenesis, and metastasis. The present work aimed to analyze the expression levels of this set of genes in tumor tissues and platelets derived from breast cancer patients by reverse transcription-quantitative polymerase chain reaction (RTqPCR) assays, in order to determine if there was an expression correlation between these sources and to take advantage of the new information to be used in possible diagnosis by liquid biopsy. Data from these assays showed that platelets and breast cancer tumors present similar expression levels of a subset of these genes\' mRNAs, depending on the molecular subtype, comorbidities, and metastasis presence.
摘要：
肿瘤微环境（TME）由高度动态的细胞群组成。每个都贡献配体，受体,可溶性蛋白质，mRNA,和miRNAs，以调节TME内的细胞活性，甚至促进血管生成或转移等过程。静脉输注的血小板（PLT）在TME中发生改变，将其转化为肿瘤培养的血小板（TEP），支持癌症的发展，血管生成,通过生物分子的脱颗粒和释放进行转移。几位作者报道了PF4，VEGF，PDGF,ANG-1,WASF3,LAPTM4B,TPM3和TAC1基因参与乳腺癌的进展，血管生成,和转移。本工作旨在通过逆转录-定量聚合酶链反应（RTqPCR）分析，分析这组基因在乳腺癌患者肿瘤组织和血小板中的表达水平。为了确定这些来源之间是否存在表达相关性，并利用新的信息来通过液体活检进行可能的诊断。这些分析的数据显示，血小板和乳腺癌肿瘤呈现相似的表达水平的这些基因的一个子集\'mRNA，根据分子亚型，合并症,和转移的存在。
