PDF(Pubmed)
Abstract：
The American mink (Neovison vison) is a semiaquatic species of Mustelid native to North America that is now widespread in China. However, the knowledge of genetic diversity of mink in China is still limited. In this study, we investigated the genetic diversity and identified significant single nucleotide polymorphisms (SNPs) in mink populations of five different color types in three different mink farms in China. Using double-digest restriction site-associated DNA sequencing, we identified a total of 1.3 million SNPs. After filtering the SNPs, phylogenetic tree, Fst, principal component, and population structure analyses were performed. The results demonstrated that red mink and black mink grouped, with separate clustering of all other color types. The population divergence index (Fst) study confirmed that different mink populations were distinct (K = 4). Two populations with different coat colors were subjected to the selection signature analysis, and 2300 genes were found to have a clear selection signature. The genes with a selection signature were subjected to Gene Ontology (GO) categorization and Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis, the results revealed that the genes with a selection signature were enriched in the melanogenesis pathway. These study\'s findings have set the stage for improved breeding and conservation of genetic resources in real-world practical mink farming.
摘要：
美国水貂（Neovisonvison）是原产于北美的一种半水生物种，现在在中国很普遍。然而,我国对水貂遗传多样性的认识还很有限。在这项研究中,我们调查了中国三个不同水貂养殖场的五种不同颜色的水貂种群的遗传多样性并鉴定了显着的单核苷酸多态性（SNPs）。使用双消化限制性位点相关DNA测序，我们确定了总共130万个SNP。过滤SNP后，系统发育树，Fst,主成分，并进行了种群结构分析。结果表明，红水貂和黑水貂分组，与所有其他颜色类型的单独聚类。种群差异指数（Fst）研究证实，不同的水貂种群是不同的（K=4）。对具有不同外套颜色的两个种群进行了选择签名分析，和2300个基因被发现有一个明确的选择特征。对具有选择特征的基因进行基因本体论（GO）分类和京都基因和基因组百科全书（KEGG）富集分析，结果显示，具有选择特征的基因在黑素生成途径中富集。这些研究的发现为在现实世界的实际水貂养殖中改善育种和遗传资源保护奠定了基础。
