PDF(Pubmed)
Abstract：
A common severe neurotoxic side effect of breast cancer (BC) therapy is chemotherapy-induced peripheral neuropathy (CIPN) and intervention is highly needed for the detection, prevention, and treatment of CIPN at an early stage. As the eye is susceptible to neurotoxic stimuli, the present study aims to determine whether CIPN signs in paclitaxel-treated BC patients correlate with ocular changes by applying advanced non-invasive biophotonic in vivo imaging. Patients (n = 14, 10 controls) underwent monitoring sessions after diagnosis, during, and after therapy (T0-T3). Monitoring sessions included general anamnesis, assessment of their quality of life, neurological scores, ophthalmological status, macular optical coherence tomography (OCT), and imaging of their subbasal nerve plexus (SNP) by large-area confocal laser-scanning microscopy (CLSM). At T0, no significant differences were detected between patients and controls. During treatment, patients\' scores significantly changed while the greatest differences were found between T0 and T3. None of the patients developed severe CIPN but retinal thickenings could be detected. CLSM revealed large SNP mosaics with identical areas while corneal nerves remained stable. The study represents the first longitudinal study combining oncological examinations with advanced biophotonic imaging techniques, demonstrating a powerful tool for the objective assessment of the severity of neurotoxic events with ocular structures acting as potential biomarkers.
摘要：
乳腺癌（BC）治疗的一个常见的严重神经毒性副作用是化疗引起的周围神经病变（CIPN），并且非常需要干预来检测。预防,以及早期CIPN的治疗。由于眼睛容易受到神经毒性刺激，本研究旨在通过应用先进的非侵入性体内生物光子成像技术,确定紫杉醇治疗的BC患者的CIPN征象是否与眼部改变相关.患者（n=14，10名对照）在诊断后接受监测，during,和治疗后（T0-T3）。监测会议包括一般回忆，评估他们的生活质量，神经学评分,眼科状态，黄斑光学相干断层扫描（OCT），并通过大面积共聚焦激光扫描显微镜（CLSM）对其基底下神经丛（SNP）进行成像。在T0时,在患者和对照之间没有检测到显著差异。治疗期间,患者的评分发生显著变化,而T0和T3之间的差异最大.没有患者出现严重的CIPN，但可以检测到视网膜增厚。CLSM显示具有相同区域的大SNP马赛克，而角膜神经保持稳定。该研究代表了第一个将肿瘤检查与先进的生物光子成像技术相结合的纵向研究。展示了一个强大的工具，用于客观评估神经毒性事件的严重程度，眼部结构作为潜在的生物标志物。
