Abstract：
In order to perform in vitro cardiotoxicity screening of pharmacological substances, multi-electrode array systems are increasingly used to measure the extracellular field potentials of cell layers of human induced pluripotent stem cell cardiomyocytes. The analysis of the field potentials is usually performed using complex analysis software provided by the hardware manufacturers. In the case of the Cardiac Analysis Tool software from Axion Biosystems, inconsistencies were found in the results, which can significantly influence the cardiotoxicity screening results. In order to obtain more reliable results, a new algorithm was developed and implemented in an easy-to-use software tool, the INCardio Data Analysis Tool, which, due to its high degree of automation, can also be used by inexperienced users. The validation reveals differences in the results of the two tools both in depolarization spike amplitudes and in the time course of the field potential durations. The manual analysis of all signals affected by deviations shows that the results of the newly developed Data Analysis Tool are correct in all cases and can therefore be classified as more accurate and reliable than the reference analysis software.
摘要：
为了进行药理物质的体外心脏毒性筛选，多电极阵列系统越来越多地用于测量人诱导多能干细胞心肌细胞的细胞层的细胞外场电位。场势的分析通常使用由硬件制造商提供的复杂分析软件来执行。在AxionBiosystems的心脏分析工具软件的情况下，结果不一致,这会显著影响心脏毒性筛查结果。为了获得更可靠的结果，在一个易于使用的软件工具中开发和实现了一种新的算法，心脏数据分析工具，which,由于其高度的自动化，也可以由没有经验的用户使用。验证揭示了两种工具在去极化尖峰幅度和场电位持续时间的时间过程中的结果差异。对所有受偏差影响的信号的手动分析表明，新开发的数据分析工具的结果在所有情况下都是正确的，因此可以归类为比参考分析软件更准确和可靠。
