Abstract：
This study aimed to investigate the effects of different slaughter methods (immersion in ice/water slurry, T1; gill cut, T2; CO2 asphyxia, T3; percussion (hit on the head with a stick), T4; Melissa officinalis L. essential oil + CO2, T5) on physiological stress, oxidative stress, and muscle quality in large yellow croaker. In terms of physiological stress, the levels of glucose (GLU), lactate dehydrogenase (LDH), and catalase (CAT) in CO2 asphyxia samples were significantly lower than those in other samples (p < 0.05). The level of cortisol (COR) in T1 sample was 1.25-1.84 times higher than that of other samples. The GLU level of T1 group was 3.2 times higher than that of T3 sample, and significantly higher than that of other samples. The creatine phosphokinase (CPK) and CAT levels of T2 samples were the highest (2.03 ng/mL and 8.34 U/mL, respectively). Furthermore, the superoxide dismutase (SOD) and glutathione peroxidase (GPx) analysis revealed that T3 and T4 samples could maintain good antioxidant enzyme activity during cold storage. The T3 samples maintained the stability of the protein (the lowest carbonyls and surface hydrophobicity) and reduced lipid oxidation (lower TBARS). In addition, the analysis of pH and water-holding capacity (WHC) revealed that T3 samples had better muscle quality. The muscle of T2 samples kept better color due to bloodletting treatment. The samples obtained after addition of Melissa officinalis L. essential oil had poorer indexes in all aspects compared to the T3 samples, which might be caused by the long anesthesia time of the essential oil.
摘要：
本研究旨在研究不同屠宰方法(浸入冰/水浆，T1;刺切,T2；CO2窒息，T3；打击乐（用棍子打在头上），T4；香蜂草精油+CO2，T5）对生理应激，氧化应激，和大黄鱼的肌肉质量。在生理压力方面，葡萄糖水平（GLU），乳酸脱氢酶（LDH），CO2窒息样品中过氧化氢酶(CAT)显著低于其他样品(p<0.05)。T1样品中的皮质醇（COR）水平是其他样品的1.25-1.84倍。T1组的GLU水平是T3组的3.2倍，并显著高于其他样品。T2样品的肌酸磷酸激酶(CPK)和CAT水平最高(2.03ng/mL和8.34U/mL,分别)。此外，超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GPx）分析表明，T3和T4样品在冷藏过程中可以保持良好的抗氧化酶活性。T3样品保持了蛋白质的稳定性(最低的羰基和表面疏水性)和降低的脂质氧化(较低的TBARS)。此外,对pH和持水量(WHC)的分析显示,T3样品具有更好的肌肉质量.T2样品的肌肉由于放血处理而保持更好的颜色。添加苦花精油后获得的样品与T3样品相比，各方面指标均较差，这可能是由于精油麻醉时间长造成的。
