PDF(Pubmed)
Abstract：
OBJECTIVE: This animal model aimed to compare the rat group that received brain irradiation and did not receive additional treatment (only saline) and the rat group that underwent brain irradiation and received Granulocyte colony stimulating factor (G-CSF) treatment. In addition, the effects of G-CSF on brain functions were examined by magnetic resonance (MR) imaging and histopathologically.
METHODS: This study used 24 female Wistar albino rats. Drug administration (saline or G-CSF) was started at the beginning of the study and continued for 15 days after whole-brain radiotherapy (WBRT). WBRT was given on day 7 of the start of the study. At the end of 15 days, the behavioral tests, including the three-chamber sociability test, open field test, and passive avoidance learning test, were done. After the behavioral test, the animals performed the MR spectroscopy procedure. At the end of the study, cervical dislocation was applied to all animals.
RESULTS: G-CSF treatment positively affected the results of the three-chamber sociability test, open-space test and passive avoidance learning test, cornu Ammonis (CA) 1, CA3, and Purkinje neuron counts, and the brain levels of brain-derived neurotrophic factor and postsynaptic density protein-95. However, G-CSF treatment reduced the glial fibrillary acidic protein immunostaining index and brain levels of malondialdehyde, tumor necrosis factor-alpha, nuclear factor kappa-B, and lactate. In addition, on MR spectroscopy, G-CSF had a reversible effect on brain lactate levels.
CONCLUSIONS: In this first designed brain irradiation animal model, which evaluated G-CSF effects, we observed that G-CSF had reparative, neuroprotective and anti-neurodegenerative effects and had increased neurotrophic factor expression, neuronal counts, and morphology changes. In addition, G-CSF had a proven lactate-lowering effect in MR spectroscopy and brain materials.
METHODS: This study used 24 female Wistar albino rats. Drug administration (saline or G-CSF) was started at the beginning of the study and continued for 15 days after whole-brain radiotherapy (WBRT). WBRT was given on day 7 of the start of the study. At the end of 15 days, the behavioral tests, including the three-chamber sociability test, open field test, and passive avoidance learning test, were done. After the behavioral test, the animals performed the MR spectroscopy procedure. At the end of the study, cervical dislocation was applied to all animals.
RESULTS: G-CSF treatment positively affected the results of the three-chamber sociability test, open-space test and passive avoidance learning test, cornu Ammonis (CA) 1, CA3, and Purkinje neuron counts, and the brain levels of brain-derived neurotrophic factor and postsynaptic density protein-95. However, G-CSF treatment reduced the glial fibrillary acidic protein immunostaining index and brain levels of malondialdehyde, tumor necrosis factor-alpha, nuclear factor kappa-B, and lactate. In addition, on MR spectroscopy, G-CSF had a reversible effect on brain lactate levels.
CONCLUSIONS: In this first designed brain irradiation animal model, which evaluated G-CSF effects, we observed that G-CSF had reparative, neuroprotective and anti-neurodegenerative effects and had increased neurotrophic factor expression, neuronal counts, and morphology changes. In addition, G-CSF had a proven lactate-lowering effect in MR spectroscopy and brain materials.
摘要：
该动物模型旨在比较接受脑照射但未接受额外治疗(仅盐水)的大鼠组和接受脑照射并接受粒细胞集落刺激因子(G-CSF)治疗的大鼠组。此外,通过磁共振成像和组织病理学检查G-CSF对脑功能的影响。
■这项研究使用了24只雌性Wistar白化病大鼠。药物施用(盐水或G-CSF)在研究开始时开始并在WBRT后持续15天。在研究开始的第7天给予WBRT。在15天结束时，行为测试，包括三室社交能力测试，露天试验,被动回避学习测试，已完成。在行为测试之后,动物进行MR光谱学程序。在研究结束时,颈椎脱位应用于所有动物。
■G-CSF治疗对三室社交能力测试的结果产生了积极影响，开放空间测试和被动回避学习测试，CA1，CA3和浦肯野神经元计数，和BDNF和PSD-95的大脑水平。然而,G-CSF治疗降低了GFAP免疫染色指数和脑内MDA水平,TNF-α,NF-kB,和乳酸。此外,关于MR光谱学，G-CSF对脑乳酸水平具有可逆作用。
■在第一个设计的脑照射动物模型中，评估了G-CSF的作用，我们观察到G-CSF具有修复性，神经保护和抗神经退行性作用，并增加了神经营养因子的表达，神经元计数，和形态变化。此外,G-CSF在MR光谱学和脑材料中具有经证实的降低乳酸的作用。
■这项研究使用了24只雌性Wistar白化病大鼠。药物施用(盐水或G-CSF)在研究开始时开始并在WBRT后持续15天。在研究开始的第7天给予WBRT。在15天结束时，行为测试，包括三室社交能力测试，露天试验,被动回避学习测试，已完成。在行为测试之后,动物进行MR光谱学程序。在研究结束时,颈椎脱位应用于所有动物。
■G-CSF治疗对三室社交能力测试的结果产生了积极影响，开放空间测试和被动回避学习测试，CA1，CA3和浦肯野神经元计数，和BDNF和PSD-95的大脑水平。然而,G-CSF治疗降低了GFAP免疫染色指数和脑内MDA水平,TNF-α,NF-kB,和乳酸。此外,关于MR光谱学，G-CSF对脑乳酸水平具有可逆作用。
■在第一个设计的脑照射动物模型中，评估了G-CSF的作用，我们观察到G-CSF具有修复性，神经保护和抗神经退行性作用，并增加了神经营养因子的表达，神经元计数，和形态变化。此外,G-CSF在MR光谱学和脑材料中具有经证实的降低乳酸的作用。
