Abstract：
OBJECTIVE: In this case-control study we aimed to investigate the intestinal microbiota profile of patients with Peutz-Jeghers syndrome (PJS) and its association with polyp growth.
METHODS: Thirty-two PJS patients and 35 healthy controls were enrolled. Fecal samples of all participants were collected for gut microbiota analysis via 16S rRNA gene (regions V3-V4) sequencing. SPSS version 22.0 and R software version 3.1.0 were used for the statistical analysis.
RESULTS: The richness was comparable, while the overall structure of the gut microbiota differed significantly between the PJS and control groups (weighted UniFrac, P = 0.001; unweighted UniFrac, P = 0.008). Significantly different abundances of two phyla, seven families, and 18 genera as well as twenty-nine differentially enriched functional modules (false discovery rate, P < 0.05) between the two groups were identified. Morganella was positively associated with the median number of polyps (JPN; r = 0.96, P < 0.001) and number of newly discovered polyps in the jejunum between two recent endoscopic resections (JPNG; r = 0.78, P = 0.04). Desulfovibrio was positively associated with JPNG (r = 0.87, P = 0.01). Blautia was negatively associated with the median maximum size of polyps in the jejunum (JPS). Anaerostipes was negatively associated with JPN, JPNG and JPS. Clostridium XVIII and Fusicatenibacter were negatively associated with JPN and JPS, respectively.
CONCLUSIONS: We found remarkably different gut microbiota of patients with PJS compared to healthy individuals and associations between specific fecal bacteria and clinical features of PJS. These findings may provide a new perspective for the management of PJS in clinical practice.
METHODS: Thirty-two PJS patients and 35 healthy controls were enrolled. Fecal samples of all participants were collected for gut microbiota analysis via 16S rRNA gene (regions V3-V4) sequencing. SPSS version 22.0 and R software version 3.1.0 were used for the statistical analysis.
RESULTS: The richness was comparable, while the overall structure of the gut microbiota differed significantly between the PJS and control groups (weighted UniFrac, P = 0.001; unweighted UniFrac, P = 0.008). Significantly different abundances of two phyla, seven families, and 18 genera as well as twenty-nine differentially enriched functional modules (false discovery rate, P < 0.05) between the two groups were identified. Morganella was positively associated with the median number of polyps (JPN; r = 0.96, P < 0.001) and number of newly discovered polyps in the jejunum between two recent endoscopic resections (JPNG; r = 0.78, P = 0.04). Desulfovibrio was positively associated with JPNG (r = 0.87, P = 0.01). Blautia was negatively associated with the median maximum size of polyps in the jejunum (JPS). Anaerostipes was negatively associated with JPN, JPNG and JPS. Clostridium XVIII and Fusicatenibacter were negatively associated with JPN and JPS, respectively.
CONCLUSIONS: We found remarkably different gut microbiota of patients with PJS compared to healthy individuals and associations between specific fecal bacteria and clinical features of PJS. These findings may provide a new perspective for the management of PJS in clinical practice.
摘要：
目的：本病例对照研究调查了Peutz-Jeghers综合征（PJS）患者的肠道菌群分布及其与息肉生长的关系。
方法：纳入32例PJS患者（P组）和35例健康对照（H组）。所有患者的临床资料，包括年龄，性别,记录息肉的数量和大小。通过16srRNA基因(区域V3-V4)测序收集所有参与者的粪便样品用于肠道微生物群分析。SPSS（版本。22.0）和R软件（版本。3.1.0)用于统计分析。
结果：丰富度相当，而PJS组和对照组的肠道微生物群的整体结构存在显着差异(加权UniFrac，P=0.001；未加权UniFrac，P=0.008)。2门的丰度明显不同，7个家庭,两组共18属。两组之间有29个差异丰富的功能模块(FDR，P<0.05)。Morganella与JPN(r=0.96,P<0.001)和JPNG(r=0.78,P=0.04)呈正相关。脱硫弧菌与JPNG呈正相关(r=0.87,P=0.01)。布劳特氏菌与JPS呈负相关(r=-0.85,P=0.02)。吻合口与JPN呈负相关(r=-0.77,P<0.04),JPNG(r=-0.88,P<0.001)和JPS(r=-0.47,P<0.04)。梭菌XVIII和镰刀菌分别与JPN(r=-0.53,P=0.002)和JPS(r=-0.50,P=0.002)呈负相关。
结论：这项研究发现，PJS患者的肠道菌群相对于健康个体存在显著差异，以及特定粪便细菌与PJS临床特征之间的关联。这些发现可能为临床实践提供新的视角。
方法：纳入32例PJS患者（P组）和35例健康对照（H组）。所有患者的临床资料，包括年龄，性别,记录息肉的数量和大小。通过16srRNA基因(区域V3-V4)测序收集所有参与者的粪便样品用于肠道微生物群分析。SPSS（版本。22.0）和R软件（版本。3.1.0)用于统计分析。
结果：丰富度相当，而PJS组和对照组的肠道微生物群的整体结构存在显着差异(加权UniFrac，P=0.001；未加权UniFrac，P=0.008)。2门的丰度明显不同，7个家庭,两组共18属。两组之间有29个差异丰富的功能模块(FDR，P<0.05)。Morganella与JPN(r=0.96,P<0.001)和JPNG(r=0.78,P=0.04)呈正相关。脱硫弧菌与JPNG呈正相关(r=0.87,P=0.01)。布劳特氏菌与JPS呈负相关(r=-0.85,P=0.02)。吻合口与JPN呈负相关(r=-0.77,P<0.04),JPNG(r=-0.88,P<0.001)和JPS(r=-0.47,P<0.04)。梭菌XVIII和镰刀菌分别与JPN(r=-0.53,P=0.002)和JPS(r=-0.50,P=0.002)呈负相关。
结论：这项研究发现，PJS患者的肠道菌群相对于健康个体存在显著差异，以及特定粪便细菌与PJS临床特征之间的关联。这些发现可能为临床实践提供新的视角。
