PDF(Pubmed)
Abstract：
Striatin is a multi-domain scaffolding protein essential for activating endothelial nitric oxide synthase (eNOS). However, its role in pre-eclampsia remains use explored. Hence, this study aimed to investigate the association between striatin and eNOS in regulating nitric oxide (NO) production in the placenta of women with and without pre-eclampsia.
Forty pregnant women each without (controls) and with pre-eclampsia (cases) were enrolled in the study. Blood striatin and NO concentrations were detected by the ELISA. Protein expression of striatin, phosphorylated eNOS (peNOS), inducible NOS (iNOS) and phosphorylated NF-κB were measured in the placental tissues by Western blot. Twenty four hour urinary protein and serum urea, uric acid and creatinine were analyzed as an autoanalyzer. Placental histology was analyzed by haematoxylin and eosin staining. Results: Compared to normotensive pregnant women, the levels of serum NO and striatin were decreased in pre-eclamptic women. The protein expression of striatin and peNOS was significantly reduced (P<0.05) while p65NF-κB and iNOS were upregulated considerably (P<0.05) in the placenta of cases compared to controls.
Our results show for the first time that decreased striatin expression was associated with decreased peNOS protein expression in the placental tissue of pre-eclamptic women. Interestingly, no significant difference was found in blood striatin or NO levels between controls and cases. Thus, therapies that improve placental striatin expression are attractive possibilities, both for prevention as well as treatment of endothelial dysfunction in pre-eclampsia.
Forty pregnant women each without (controls) and with pre-eclampsia (cases) were enrolled in the study. Blood striatin and NO concentrations were detected by the ELISA. Protein expression of striatin, phosphorylated eNOS (peNOS), inducible NOS (iNOS) and phosphorylated NF-κB were measured in the placental tissues by Western blot. Twenty four hour urinary protein and serum urea, uric acid and creatinine were analyzed as an autoanalyzer. Placental histology was analyzed by haematoxylin and eosin staining. Results: Compared to normotensive pregnant women, the levels of serum NO and striatin were decreased in pre-eclamptic women. The protein expression of striatin and peNOS was significantly reduced (P<0.05) while p65NF-κB and iNOS were upregulated considerably (P<0.05) in the placenta of cases compared to controls.
Our results show for the first time that decreased striatin expression was associated with decreased peNOS protein expression in the placental tissue of pre-eclamptic women. Interestingly, no significant difference was found in blood striatin or NO levels between controls and cases. Thus, therapies that improve placental striatin expression are attractive possibilities, both for prevention as well as treatment of endothelial dysfunction in pre-eclampsia.
摘要：
■纹状体蛋白是激活内皮型一氧化氮合酶（eNOS）所必需的多结构域支架蛋白。然而,其在先兆子痫中的作用仍在探索中。因此，本研究旨在探讨纹状体蛋白和eNOS在调节先兆子痫妇女胎盘一氧化氮（NO）产生中的关系。
■40名无先兆子痫（对照）和有先兆子痫（病例）的孕妇被纳入研究。通过ELISA检测血液中的纹状体和NO浓度。纹状体蛋白的表达，磷酸化eNOS（peNOS），采用蛋白质印迹法检测胎盘组织中诱导型NOS(iNOS)和磷酸化NF-κB。24小时尿蛋白和血清尿素，尿酸和肌酐作为自动分析仪进行分析.通过苏木精和伊红染色分析胎盘组织学。
■与血压正常的孕妇相比，先兆子痫妇女的血清NO和纹状体蛋白水平降低。与对照组相比，病例胎盘中纹状体蛋白和peNOS的蛋白表达显着降低（P<0.05），而p65NF-κB和iNOS的表达明显上调（P<0.05）。
■我们的结果首次表明，在先兆子痫妇女的胎盘组织中，纹状体蛋白表达减少与peNOS蛋白表达减少有关。有趣的是,对照组和病例之间的血纹状体蛋白或NO水平没有显着差异。因此，改善胎盘纹状体蛋白表达的疗法是有吸引力的可能性，用于预防和治疗先兆子痫的内皮功能障碍。
■40名无先兆子痫（对照）和有先兆子痫（病例）的孕妇被纳入研究。通过ELISA检测血液中的纹状体和NO浓度。纹状体蛋白的表达，磷酸化eNOS（peNOS），采用蛋白质印迹法检测胎盘组织中诱导型NOS(iNOS)和磷酸化NF-κB。24小时尿蛋白和血清尿素，尿酸和肌酐作为自动分析仪进行分析.通过苏木精和伊红染色分析胎盘组织学。
■与血压正常的孕妇相比，先兆子痫妇女的血清NO和纹状体蛋白水平降低。与对照组相比，病例胎盘中纹状体蛋白和peNOS的蛋白表达显着降低（P<0.05），而p65NF-κB和iNOS的表达明显上调（P<0.05）。
■我们的结果首次表明，在先兆子痫妇女的胎盘组织中，纹状体蛋白表达减少与peNOS蛋白表达减少有关。有趣的是,对照组和病例之间的血纹状体蛋白或NO水平没有显着差异。因此，改善胎盘纹状体蛋白表达的疗法是有吸引力的可能性，用于预防和治疗先兆子痫的内皮功能障碍。
