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Abstract：
BACKGROUND: Plants are able to deliver a huge number of differing bioactive compounds which may supplement the requirements of the human body by acting as natural antioxidants. Antioxidants are mindful for the defense component of the life form against the pathologies related to the assault of free radicals. The main purpose of this study was to investigate the qualitative phytochemical composition of Vernonia amygdalina leaf extract and its antioxidant activity.
METHODS: The powdered plant sample was successively extracted with aqueous, methanol and ethanol solvents using Soxhlet apparatus. The antioxidant activities of the crude leaf extract were determined using 1, 1- diphenyl-2-picryl hydrazyl (DPPH) radical, 2,2\'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, phosphomolybdate (PM) and hydrogen peroxide (H2O2) scavenging assay. All the examinations were drained triplicates and average values of each test were taken.
RESULTS: Phytochemical investigation of the plant revealed that the three solvent extracts contained numerous bioactive compounds namely alkaloids, tannins, saponins, phenols, terpenoids, steroids, glycosides and sugars. The result showed that, the leaf extracts of V. amygdalina obtained from methanol extract exhibit the maximum antioxidant activity compared ethanol and aqueous extracts. The IC50 values of DPPH assay for the H2O, MeOH and EtOH extracts were 111.4, 94.92 and 94.83 μg/ml; of ABTS assay were 334.3, 179.8 and 256.9 μg/ml; of H2O2 assay were 141.6, 156 and 180.6 μg/ml, respectively. The maximum radical scavenging activity was obtained in DPPH assay while the lowest scavenging activity was obtained in ABTS assay method. The data obtained in the in vitro models clearly suggest that methanol extract has higher antioxidant activity due to a higher presence of phenolic constituents in the extract.
CONCLUSIONS: This study revealed that V. amygdalina leaf has a noteworthy antioxidant and free radical scavenging activity mitigating the traditional use of the plant for different aliments.
METHODS: The powdered plant sample was successively extracted with aqueous, methanol and ethanol solvents using Soxhlet apparatus. The antioxidant activities of the crude leaf extract were determined using 1, 1- diphenyl-2-picryl hydrazyl (DPPH) radical, 2,2\'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) radical, phosphomolybdate (PM) and hydrogen peroxide (H2O2) scavenging assay. All the examinations were drained triplicates and average values of each test were taken.
RESULTS: Phytochemical investigation of the plant revealed that the three solvent extracts contained numerous bioactive compounds namely alkaloids, tannins, saponins, phenols, terpenoids, steroids, glycosides and sugars. The result showed that, the leaf extracts of V. amygdalina obtained from methanol extract exhibit the maximum antioxidant activity compared ethanol and aqueous extracts. The IC50 values of DPPH assay for the H2O, MeOH and EtOH extracts were 111.4, 94.92 and 94.83 μg/ml; of ABTS assay were 334.3, 179.8 and 256.9 μg/ml; of H2O2 assay were 141.6, 156 and 180.6 μg/ml, respectively. The maximum radical scavenging activity was obtained in DPPH assay while the lowest scavenging activity was obtained in ABTS assay method. The data obtained in the in vitro models clearly suggest that methanol extract has higher antioxidant activity due to a higher presence of phenolic constituents in the extract.
CONCLUSIONS: This study revealed that V. amygdalina leaf has a noteworthy antioxidant and free radical scavenging activity mitigating the traditional use of the plant for different aliments.
摘要：
背景：植物能够提供大量不同的生物活性化合物，这些化合物可以通过充当天然抗氧化剂来补充人体的需求。抗氧化剂注意生命形式的防御部分，以抵抗与自由基攻击有关的病理。本研究的主要目的是研究杏仁叶提取物的定性植物化学成分及其抗氧化活性。
方法：用水溶液连续提取粉状植物样品，使用索氏装置的甲醇和乙醇溶剂。使用1，1-二苯基-2-吡啶酰肼（DPPH）自由基测定粗叶提取物的抗氧化活性，2,2'-氮杂-双(3-乙基苯并噻唑啉-6-磺酸(ABTS)基团,磷钼酸盐(PM)和过氧化氢(H2O2)清除测定。所有检查均一式三份引流，并获取每个测试的平均值。
结果：对植物的植物化学研究表明，三种溶剂提取物含有许多生物活性化合物，即生物碱，单宁,皂苷,酚类物质,萜类化合物,类固醇,糖苷和糖。结果表明,与乙醇和水提取物相比，从甲醇提取物中获得的V.Amgdalina叶提取物表现出最大的抗氧化活性。DPPH测定对H2O的IC50值，MeOH和EtOH提取物为111.4、94.92和94.83μg/ml；ABTS测定为334.3、179.8和256.9μg/ml；H2O2测定为141.6、156和180.6μg/ml，分别。在DPPH试验中获得了最大的自由基清除活性，而在ABTS试验方法中获得了最低的清除活性。在体外模型中获得的数据清楚地表明，由于提取物中酚类成分的含量较高，甲醇提取物具有较高的抗氧化活性。
结论：这项研究表明，杏仁核叶具有值得注意的抗氧化和自由基清除活性，从而减轻了植物在不同营养中的传统用途。
方法：用水溶液连续提取粉状植物样品，使用索氏装置的甲醇和乙醇溶剂。使用1，1-二苯基-2-吡啶酰肼（DPPH）自由基测定粗叶提取物的抗氧化活性，2,2'-氮杂-双(3-乙基苯并噻唑啉-6-磺酸(ABTS)基团,磷钼酸盐(PM)和过氧化氢(H2O2)清除测定。所有检查均一式三份引流，并获取每个测试的平均值。
结果：对植物的植物化学研究表明，三种溶剂提取物含有许多生物活性化合物，即生物碱，单宁,皂苷,酚类物质,萜类化合物,类固醇,糖苷和糖。结果表明,与乙醇和水提取物相比，从甲醇提取物中获得的V.Amgdalina叶提取物表现出最大的抗氧化活性。DPPH测定对H2O的IC50值，MeOH和EtOH提取物为111.4、94.92和94.83μg/ml；ABTS测定为334.3、179.8和256.9μg/ml；H2O2测定为141.6、156和180.6μg/ml，分别。在DPPH试验中获得了最大的自由基清除活性，而在ABTS试验方法中获得了最低的清除活性。在体外模型中获得的数据清楚地表明，由于提取物中酚类成分的含量较高，甲醇提取物具有较高的抗氧化活性。
结论：这项研究表明，杏仁核叶具有值得注意的抗氧化和自由基清除活性，从而减轻了植物在不同营养中的传统用途。
