PDF(Pubmed)
Abstract：
Investigating genomic regions associated with morphometric traits in camels is valuable, because it allows a better understanding of adaptive and productive features to implement a sustainable management and a customised breeding program for dromedaries.
With a genome-wide association study (GWAS) including 96 Iranian dromedaries phenotyped for 12 morphometric traits and genotyped-by-sequencing (GBS) with 14,522 SNPs, we aimed at identifying associated candidate genes.
The association between SNPs and morphometric traits was investigated using a linear mixed model with principal component analysis (PCA) and kinship matrix.
With this approach, we detected 59 SNPs located in 37 candidate genes potentially associated to morphometric traits in dromedaries. The top associated SNPs were related to pin width, whither to pin length, height at whither, muzzle girth, and tail length. Interestingly, the results highlight the association between whither height, muzzle circumference, tail length, whither to pin length. The identified candidate genes were associated with growth, body size, and immune system in other species.
We identified three key hub genes in the gene network analysis including ACTB, SOCS1 and ARFGEF1. In the central position of gene network, ACTB was detected as the most important gene related to muscle function. With this initial GWAS using GBS on dromedary camels for morphometric traits, we show that this SNP panel can be effective for genetic evaluation of growth in dromedaries. However, we suggest a higher-density SNP array may greatly improve the reliability of the results.
With a genome-wide association study (GWAS) including 96 Iranian dromedaries phenotyped for 12 morphometric traits and genotyped-by-sequencing (GBS) with 14,522 SNPs, we aimed at identifying associated candidate genes.
The association between SNPs and morphometric traits was investigated using a linear mixed model with principal component analysis (PCA) and kinship matrix.
With this approach, we detected 59 SNPs located in 37 candidate genes potentially associated to morphometric traits in dromedaries. The top associated SNPs were related to pin width, whither to pin length, height at whither, muzzle girth, and tail length. Interestingly, the results highlight the association between whither height, muzzle circumference, tail length, whither to pin length. The identified candidate genes were associated with growth, body size, and immune system in other species.
We identified three key hub genes in the gene network analysis including ACTB, SOCS1 and ARFGEF1. In the central position of gene network, ACTB was detected as the most important gene related to muscle function. With this initial GWAS using GBS on dromedary camels for morphometric traits, we show that this SNP panel can be effective for genetic evaluation of growth in dromedaries. However, we suggest a higher-density SNP array may greatly improve the reliability of the results.
摘要：
背景：研究与骆驼形态特征相关的基因组区域是有价值的，因为它可以更好地了解适应性和生产性功能，以实施可持续管理和单峰控制的定制育种计划。
目的：通过全基因组关联研究（GWAS），包括96个伊朗单峰体，其表型为12个形态特征，并通过测序（GBS）进行基因分型，具有14,522个SNP，我们旨在确定相关的候选基因。
方法：使用具有主成分分析（PCA）和亲缘关系矩阵的线性混合模型研究了SNP与形态测量性状之间的关联。
结果：通过这种方法，我们检测到位于37个候选基因中的59个SNPs,这些基因可能与单峰动物的形态特征相关.顶部相关的SNP与pin宽度有关，到销长度，到哪里的高度，枪口周长，和尾巴长度。有趣的是,结果突出了身高之间的关联，枪口圆周,尾部长度,到销长度。确定的候选基因与生长有关，身体尺寸,和其他物种的免疫系统。
结论：我们在基因网络分析中确定了三个关键的枢纽基因，包括ACTB，SOCS1和ARFGEF1。在基因网络的中心位置，ACTB被检测为与肌肉功能相关的最重要的基因。在这个最初的GWAS中，使用单峰骆驼的GBS来研究形态特征，我们表明，该SNP面板可以有效地对单峰动物的生长进行遗传评估。然而,我们建议使用更高密度的SNP阵列可以大大提高结果的可靠性.
目的：通过全基因组关联研究（GWAS），包括96个伊朗单峰体，其表型为12个形态特征，并通过测序（GBS）进行基因分型，具有14,522个SNP，我们旨在确定相关的候选基因。
方法：使用具有主成分分析（PCA）和亲缘关系矩阵的线性混合模型研究了SNP与形态测量性状之间的关联。
结果：通过这种方法，我们检测到位于37个候选基因中的59个SNPs,这些基因可能与单峰动物的形态特征相关.顶部相关的SNP与pin宽度有关，到销长度，到哪里的高度，枪口周长，和尾巴长度。有趣的是,结果突出了身高之间的关联，枪口圆周,尾部长度,到销长度。确定的候选基因与生长有关，身体尺寸,和其他物种的免疫系统。
结论：我们在基因网络分析中确定了三个关键的枢纽基因，包括ACTB，SOCS1和ARFGEF1。在基因网络的中心位置，ACTB被检测为与肌肉功能相关的最重要的基因。在这个最初的GWAS中，使用单峰骆驼的GBS来研究形态特征，我们表明，该SNP面板可以有效地对单峰动物的生长进行遗传评估。然而,我们建议使用更高密度的SNP阵列可以大大提高结果的可靠性.
