PDF(Pubmed)
Abstract：
OBJECTIVE: To investigate whether high relative humidity conditions (HC), when using a time-lapse system (TLS) with sequential culture media, are beneficial to embryo culture, improving ongoing pregnancy rates.
METHODS: We included patients undergoing their first ICSI cycle treatment from April 2021 to May 2022. Patients assigned to dry conditions (DC) or HC were 278 and 218, respectively. We used a GERI TLS, three chambers configured in humidity conditions and three in dry conditions. The effect of HC on ongoing pregnancy rate was assessed by the propensity matched sample, to reduce potential differences between women undergoing either HC or DC and reduce biased estimation of treatment effect.
RESULTS: After adjusting for several confounding variables and applying the propensity score (PS), no significant differences were observed in the rates of normal (2PN) and abnormal (1PN and 3PN) fertilization, blastulation, top-quality blastocysts, frozen blastocysts, ongoing pregnancies, and miscarriages. The 2-cell (t2) and 4-cell (t4) stages and cell divisions between such stages occurred earlier and were more synchronous in the in DC.
CONCLUSIONS: These results suggest that HC conditions do not improve the rate of ongoing pregnancy and several embryological outcomes, under the conditions used in this study based on a time-lapse system and sequential culture with day 3 medium change-over.
METHODS: We included patients undergoing their first ICSI cycle treatment from April 2021 to May 2022. Patients assigned to dry conditions (DC) or HC were 278 and 218, respectively. We used a GERI TLS, three chambers configured in humidity conditions and three in dry conditions. The effect of HC on ongoing pregnancy rate was assessed by the propensity matched sample, to reduce potential differences between women undergoing either HC or DC and reduce biased estimation of treatment effect.
RESULTS: After adjusting for several confounding variables and applying the propensity score (PS), no significant differences were observed in the rates of normal (2PN) and abnormal (1PN and 3PN) fertilization, blastulation, top-quality blastocysts, frozen blastocysts, ongoing pregnancies, and miscarriages. The 2-cell (t2) and 4-cell (t4) stages and cell divisions between such stages occurred earlier and were more synchronous in the in DC.
CONCLUSIONS: These results suggest that HC conditions do not improve the rate of ongoing pregnancy and several embryological outcomes, under the conditions used in this study based on a time-lapse system and sequential culture with day 3 medium change-over.
摘要：
目的：为了研究高相对湿度条件（HC）当使用带有顺序培养基的延时系统（TLS）时，有利于胚胎培养，提高怀孕率。
方法：我们纳入了2021年4月至2022年5月接受第一个ICSI周期治疗的患者。被分配到干燥条件（DC）或HC的患者分别为278和218。我们使用了GERITLS,三个室配置在湿度条件和三个在干燥条件。通过倾向匹配样本评估HC对持续妊娠率的影响，减少接受HC或DC的女性之间的潜在差异，并减少对治疗效果的偏倚估计。
结果：在调整了几个混杂变量并应用倾向评分（PS）之后，正常(2PN)和异常(1PN和3PN)受精率无显著差异,囊胚形成,高质量的胚泡，冷冻胚泡,正在怀孕,和流产。2细胞(t2)和4细胞(t4)阶段和这些阶段之间的细胞分裂发生得更早,并且在DC中更同步。
结论：这些结果表明，HC条件不能改善持续妊娠率和一些胚胎结局，在本研究中使用的条件下，基于延时系统和第3天培养基转换的顺序培养。
方法：我们纳入了2021年4月至2022年5月接受第一个ICSI周期治疗的患者。被分配到干燥条件（DC）或HC的患者分别为278和218。我们使用了GERITLS,三个室配置在湿度条件和三个在干燥条件。通过倾向匹配样本评估HC对持续妊娠率的影响，减少接受HC或DC的女性之间的潜在差异，并减少对治疗效果的偏倚估计。
结果：在调整了几个混杂变量并应用倾向评分（PS）之后，正常(2PN)和异常(1PN和3PN)受精率无显著差异,囊胚形成,高质量的胚泡，冷冻胚泡,正在怀孕,和流产。2细胞(t2)和4细胞(t4)阶段和这些阶段之间的细胞分裂发生得更早,并且在DC中更同步。
结论：这些结果表明，HC条件不能改善持续妊娠率和一些胚胎结局，在本研究中使用的条件下，基于延时系统和第3天培养基转换的顺序培养。
