Abstract：
BACKGROUND: Pseudomonas aeruginosa is an opportunistic gram-negative pathogen with multiple mechanisms of resistance to antibiotics. This systematic review aimed to study the antibacterial effects of nanocomposites on efflux pump expression and biofilm production in P. aeruginosa.
METHODS: The search was conducted from January 1, 2000, to May 30, 2022, using terms such as (P. aeruginosa) AND (biofilm) AND (antibiofilm activity) AND (anti-Efflux Pump Expression activity) AND (nanoparticles) AND (Efflux Pump Expression) AND (Solid Lipid NPS) AND (Nano Lipid Carriers). Many databases are included in the collection, including ScienceDirect, PubMed, Scopus, Ovid, and Cochrane.
RESULTS: A list of selected articles was retrieved by using the relevant keywords. A total of 323 published papers were selected and imported into the Endnote library (version X9). Following the removal of duplicates, 240 were selected for further processing. Based on the titles and abstracts of the articles, 54 irrelevant studies were excluded. Among the remaining 186 articles, 54 were included in the analysis because their full texts were accessible. Ultimately, 74 studies were selected based on inclusion/exclusion criteria.
CONCLUSIONS: Recent studies regarding the impact of NPs on drug resistance in P. aeruginosa found that various nanostructures were developed with different antimicrobial properties. The results of our study suggest that NPs may be a feasible alternative for combating microbial resistance in P. aeruginosa by blocking flux pumps and inhibiting biofilm formation.
METHODS: The search was conducted from January 1, 2000, to May 30, 2022, using terms such as (P. aeruginosa) AND (biofilm) AND (antibiofilm activity) AND (anti-Efflux Pump Expression activity) AND (nanoparticles) AND (Efflux Pump Expression) AND (Solid Lipid NPS) AND (Nano Lipid Carriers). Many databases are included in the collection, including ScienceDirect, PubMed, Scopus, Ovid, and Cochrane.
RESULTS: A list of selected articles was retrieved by using the relevant keywords. A total of 323 published papers were selected and imported into the Endnote library (version X9). Following the removal of duplicates, 240 were selected for further processing. Based on the titles and abstracts of the articles, 54 irrelevant studies were excluded. Among the remaining 186 articles, 54 were included in the analysis because their full texts were accessible. Ultimately, 74 studies were selected based on inclusion/exclusion criteria.
CONCLUSIONS: Recent studies regarding the impact of NPs on drug resistance in P. aeruginosa found that various nanostructures were developed with different antimicrobial properties. The results of our study suggest that NPs may be a feasible alternative for combating microbial resistance in P. aeruginosa by blocking flux pumps and inhibiting biofilm formation.
摘要：
背景：铜绿假单胞菌是一种机会性革兰氏阴性病原体，对抗生素具有多种耐药机制。本系统综述旨在研究纳米复合材料对铜绿假单胞菌外排泵表达和生物膜产生的抗菌作用。
方法：搜索是从2000年1月1日至2022年5月30日进行的，使用诸如（P。铜绿假单胞菌）和（生物膜）和（抗生物膜活性）和（抗外排泵表达活性）和（纳米颗粒）和（外排泵表达）和（固体脂质NPS）和（纳米脂质载体）。集合中包含许多数据库，包括ScienceDirect,PubMed,Scopus,奥维德,还有Cochrane.
结果：通过使用相关关键字检索选定的文章列表。总共选择了323篇已发表的论文，并将其导入到Endnote图书馆（X9版）中。删除重复项之后，选择240进行进一步处理。根据文章的标题和摘要，54项无关研究被排除在外。在剩下的186篇文章中，54个被纳入分析，因为它们的全文是可访问的。最终,根据纳入/排除标准选择了74项研究。
结论：关于NPs对铜绿假单胞菌耐药性影响的最新研究发现，开发出具有不同抗菌特性的各种纳米结构。我们的研究结果表明，NP可能是通过阻断通量泵和抑制生物膜形成来对抗铜绿假单胞菌微生物耐药性的可行替代方法。
方法：搜索是从2000年1月1日至2022年5月30日进行的，使用诸如（P。铜绿假单胞菌）和（生物膜）和（抗生物膜活性）和（抗外排泵表达活性）和（纳米颗粒）和（外排泵表达）和（固体脂质NPS）和（纳米脂质载体）。集合中包含许多数据库，包括ScienceDirect,PubMed,Scopus,奥维德,还有Cochrane.
结果：通过使用相关关键字检索选定的文章列表。总共选择了323篇已发表的论文，并将其导入到Endnote图书馆（X9版）中。删除重复项之后，选择240进行进一步处理。根据文章的标题和摘要，54项无关研究被排除在外。在剩下的186篇文章中，54个被纳入分析，因为它们的全文是可访问的。最终,根据纳入/排除标准选择了74项研究。
结论：关于NPs对铜绿假单胞菌耐药性影响的最新研究发现，开发出具有不同抗菌特性的各种纳米结构。我们的研究结果表明，NP可能是通过阻断通量泵和抑制生物膜形成来对抗铜绿假单胞菌微生物耐药性的可行替代方法。
