Abstract：
BACKGROUND: Research on the genetic mechanisms of hypertension has been a hot topic in the cardiovascular field.
OBJECTIVE: To study the correlation between senile hypertension and traditional Chinese medicine (TCM) constitution and lipoprotein lipase (LPL) gene polymorphism and to provide the theoretical basis for TCM prevention and treatment of hypertension.
METHODS: The elderly population in communities in Shanghai (hypertensive: 264 cases; non-hypertensive: 159 cases) was taken as the research object. Essential data and information on TCM constitution were collected. The LPL gene mutation was detected using the second-generation sequencing method. Statistical analysis was performed to clarify the relationship between hypertension and senile hypertension. The correlation of TCM constitution with risk factors and LPL gene polymorphisms was studied.
RESULTS: The primary TCM constitutions in the hypertension group were phlegm-dampness constitution (51.52%), yin-deficiency constitution (17.42%), balanced constitution (15.53%), and yin-deficiency (9.43%). Logistic regression analysis showed that the phlegm-dampness constitution (P< 0.05, OR = 2.587) and yin-deficiency constitution (P< 0.01, OR = 2.693) were the risk constitutions of hypertension in the elderly. A total of 37 LPL gene mutation loci (SNP: 22; new discovery: 15) were detected in the LPL gene, and the mutation rates of rs254, rs255, rs3208305, rs316, rs11570891, rs328, rs11570893, and rs13702 were relatively high, which were 26.24%, 26.24%, 16.08%, 14.66%, 13.24%, 12.06%, and 10.64%. In the phlegm-dampness group, the proportion of rs254 CC type, rs255 TT type, and rs13702 TT type in the hypertensive group (77.21%, 77.21%, and 93.38%) was higher than that in the non-hypertensive group (56.41%, 56.41%, and 82.05%), The difference was statistically significant (P< 0.05).
CONCLUSIONS: The phlegm-dampness constitution and yin-deficiency constitution are the risk factors of hypertension in the elderly; in the phlegm-dampness population, rs254 CC type, rs255 TT type, and rs13702 TT type are the risk factors for elderly hypertension.
OBJECTIVE: To study the correlation between senile hypertension and traditional Chinese medicine (TCM) constitution and lipoprotein lipase (LPL) gene polymorphism and to provide the theoretical basis for TCM prevention and treatment of hypertension.
METHODS: The elderly population in communities in Shanghai (hypertensive: 264 cases; non-hypertensive: 159 cases) was taken as the research object. Essential data and information on TCM constitution were collected. The LPL gene mutation was detected using the second-generation sequencing method. Statistical analysis was performed to clarify the relationship between hypertension and senile hypertension. The correlation of TCM constitution with risk factors and LPL gene polymorphisms was studied.
RESULTS: The primary TCM constitutions in the hypertension group were phlegm-dampness constitution (51.52%), yin-deficiency constitution (17.42%), balanced constitution (15.53%), and yin-deficiency (9.43%). Logistic regression analysis showed that the phlegm-dampness constitution (P< 0.05, OR = 2.587) and yin-deficiency constitution (P< 0.01, OR = 2.693) were the risk constitutions of hypertension in the elderly. A total of 37 LPL gene mutation loci (SNP: 22; new discovery: 15) were detected in the LPL gene, and the mutation rates of rs254, rs255, rs3208305, rs316, rs11570891, rs328, rs11570893, and rs13702 were relatively high, which were 26.24%, 26.24%, 16.08%, 14.66%, 13.24%, 12.06%, and 10.64%. In the phlegm-dampness group, the proportion of rs254 CC type, rs255 TT type, and rs13702 TT type in the hypertensive group (77.21%, 77.21%, and 93.38%) was higher than that in the non-hypertensive group (56.41%, 56.41%, and 82.05%), The difference was statistically significant (P< 0.05).
CONCLUSIONS: The phlegm-dampness constitution and yin-deficiency constitution are the risk factors of hypertension in the elderly; in the phlegm-dampness population, rs254 CC type, rs255 TT type, and rs13702 TT type are the risk factors for elderly hypertension.
摘要：
背景：高血压的遗传机制一直是心血管领域的研究热点。
目的：研究老年高血压与中医体质及脂蛋白脂酶(LPL)基因多态性的相关性，为中医防治高血压提供理论依据。
方法：以上海市社区老年人群（高血压264例，非高血压159例）为研究对象。收集中医体质的基本数据和信息。使用第二代测序方法检测LPL基因突变。进行统计学分析以阐明高血压与老年高血压的关系。研究中医体质与危险因素及LPL基因多态性的相关性。
结果:高血压组中医体质主要为痰湿体质(51.52%),阴虚体质(17.42%)，均衡体质（15.53%），阴虚(9.43%)。Logistic回归分析显示,痰湿体质(P<0.05,OR=2.587)和阴虚体质(P<0.01,OR=2.693)是老年人高血压的危险体质。共检测到37个LPL基因突变位点(SNP:22;新发现:15),rs254,rs255,rs3208305,rs316,rs11570891,rs328,rs11570893,rs13702的突变率较高,占26.24%，26.24%,16.08%,14.66%,13.24%,12.06%,和10.64%。在痰湿组中，RS254CC型的比例，rs255TT型，高血压组rs13702TT型（77.21%，77.21%,和93.38％）高于非高血压组（56.41％，56.41%，和82.05%），差异有统计学意义(P<0.05)。
结论:痰湿体质和阴虚体质是中老年人高血压的危险因素;在痰湿人群中,rs254CC型，rs255TT型，rs13702TT型是老年高血压的危险因素。
目的：研究老年高血压与中医体质及脂蛋白脂酶(LPL)基因多态性的相关性，为中医防治高血压提供理论依据。
方法：以上海市社区老年人群（高血压264例，非高血压159例）为研究对象。收集中医体质的基本数据和信息。使用第二代测序方法检测LPL基因突变。进行统计学分析以阐明高血压与老年高血压的关系。研究中医体质与危险因素及LPL基因多态性的相关性。
结果:高血压组中医体质主要为痰湿体质(51.52%),阴虚体质(17.42%)，均衡体质（15.53%），阴虚(9.43%)。Logistic回归分析显示,痰湿体质(P<0.05,OR=2.587)和阴虚体质(P<0.01,OR=2.693)是老年人高血压的危险体质。共检测到37个LPL基因突变位点(SNP:22;新发现:15),rs254,rs255,rs3208305,rs316,rs11570891,rs328,rs11570893,rs13702的突变率较高,占26.24%，26.24%,16.08%,14.66%,13.24%,12.06%,和10.64%。在痰湿组中，RS254CC型的比例，rs255TT型，高血压组rs13702TT型（77.21%，77.21%,和93.38％）高于非高血压组（56.41％，56.41%，和82.05%），差异有统计学意义(P<0.05)。
结论:痰湿体质和阴虚体质是中老年人高血压的危险因素;在痰湿人群中,rs254CC型，rs255TT型，rs13702TT型是老年高血压的危险因素。
