Abstract：
The induction of vasculature formation is proposed to be a significant mechanism behind the non-genotoxic carcinogenicity of a chemical. The vasculature formation model used in this study is based on the coculture of human primary HUVECs and hASCs. This model was used to develop an assay to assess the induction of vasculature formation. Three assay protocols, based on different conditions, were developed and compared in order to identify the optimal conditions required. Some serum supplements and growth factors were observed to be essential for initiating vasculature formation. Of the studied putative positive reference chemicals, aspartame, sodium nitrite, bisphenol A and nicotine treatment led to a clear induction of vasculature formation, but arsenic and cadmium treatment only led to a slight increase. This human cell-based assay has the potential to be used as one test within a next generation testing battery, to assess the non-genotoxic carcinogenicity of a chemical through the mechanism of vasculature formation induction.
摘要：
血管形成的诱导被认为是化学物质非遗传毒性致癌性背后的重要机制。本研究中使用的脉管系统形成模型基于人原代HUVEC和hASC的共培养。该模型用于开发评估脉管系统形成的诱导的测定。三个检测方案,基于不同的条件，进行了开发和比较，以确定所需的最佳条件。观察到一些血清补充剂和生长因子对于启动脉管系统形成是必需的。在研究的假定阳性参考化学品中，阿斯巴甜,亚硝酸钠,双酚A和尼古丁治疗导致血管形成的明显诱导，但砷和镉处理仅导致轻微增加。这种基于人类细胞的测定法有可能用作下一代测试电池中的一种测试，通过血管形成诱导机制评估化学物质的非遗传毒性致癌性。
