PDF(Pubmed)
Abstract：
Sargassum horneri (S. horneri) is a brown seaweed that contains a fucose-rich sulfated polysaccharide called fucoidan and is known to possess beneficial bioactivities, such as anti-inflammatory, antiviral, antioxidative, and antitumoral effects. This study aimed to determine the anti-inflammatory effects of AB_SH (hydrothermal extracts from S. horneri) and its bioactive compound (fucoidan) against tumor necrosis factor alpha (TNF-α)-induced inflammation in human retinal pigment epithelial (RPE) cells. AB_SH did not exhibit any cytotoxicity, and it decreased the mRNA expression of interleukin (IL)-6 and IL-8 and the production of the cytokines IL-6 and TNF-α. It also suppressed the expression levels of phosphorylated nuclear factor kappa B (NF-κB) and mitogen-activated protein kinases (MAPKs), including c-Jun amino-terminal kinases (JNK), p38 protein kinases (p38), and extracellular signal-regulated kinase (ERK) proteins, suggesting that AB_SH inhibits activation of the NF-kB/MAPK signaling pathway. Since fucoidan was identified in the composition analysis of AB_SH, it was additionally shown to be required for its anti-inflammatory effects in TNF-α-stimulated human RPE cells. In line with the AB_SH results, fucoidan reduced the mRNA levels of IL-6, IL-1ß, and IL-8 and production of the cytokines IL-6, TNF-α, and IL-8 through the downregulation of the NF-kB/MAPK signaling pathway in a dose-dependent manner. Collectively, the ability of AB_SH from S. horneri hydrothermal extracts to reduce inflammation indicates that it may be a good functional ingredient for managing ocular disorders.
摘要：
马尾藻(S.horneri）是一种棕色海藻，含有富含岩藻糖的硫酸多糖，称为岩藻依聚糖，并且已知具有有益的生物活性，如抗炎，抗病毒，抗氧化，和抗肿瘤作用。本研究旨在确定AB_SH（S.horneri的水热提取物）及其生物活性化合物（岩藻依聚糖）对人视网膜色素上皮（RPE）细胞肿瘤坏死因子α（TNF-α）诱导的炎症的抗炎作用。AB_SH没有表现出任何细胞毒性，它降低了白细胞介素（IL）-6和IL-8的mRNA表达以及细胞因子IL-6和TNF-α的产生。它还抑制磷酸化核因子κB（NF-κB）和丝裂原活化蛋白激酶（MAPK）的表达水平，包括c-Jun氨基末端激酶（JNK），p38蛋白激酶（p38），和细胞外信号调节激酶（ERK）蛋白，提示AB_SH抑制NF-kB/MAPK信号通路的激活。由于在AB_SH的组成分析中鉴定了岩藻依聚糖，此外，它在TNF-α刺激的人RPE细胞中的抗炎作用是必需的。与AB_SH结果一致，岩藻依聚糖降低IL-6，IL-1β的mRNA水平，和IL-8和细胞因子IL-6，TNF-α的产生，和IL-8通过下调NF-kB/MAPK信号通路呈剂量依赖性。总的来说，来自S.horneri热液提取物的AB_SH减少炎症的能力表明它可能是管理眼部疾病的良好功能成分。
