PDF(Pubmed)
Abstract：
Rapeseed (Brassica napus L.), the third largest oil crop, is an important source of vegetable oil and biofuel for the world. Although the breeding and yield has been improved, rapeseed still has the lowest yield compared with other major crops. Thus, increasing rapeseed yield is essential for the high demand of vegetable oil and high-quality protein for live stocks. Silique number per plant (SN), seed per pod (SP), and 1000-seed weight (SW) are the three important factors for seed yield in rapeseed. Some yield-related traits, including plant height (PH), flowering time (FT), primary branch number (BN) and silique number per inflorescence (SI) also affect the yield per plant (YP). Using six multi-locus genome-wide association study (ML-GWAS) approaches, a total of 908 yield-related quantitative trait nucleotides (QTNs) were identified in a panel consisting of 403 rapeseed core accessions based on whole-genome sequencing. Integration of ML-GWAS with transcriptome analysis, 79 candidate genes, including BnaA09g39790D (RNA helicase), BnaA09g39950D (Lipase) and BnaC09g25980D (SWEET7), were further identified and twelve genes were validated by qRT-PCRs to affect the SW or SP in rapeseed. The distribution of superior alleles from nineteen stable QTNs in 20 elite rapeseed accessions suggested that the high-yielding accessions contained more superior alleles. These results would contribute to a further understanding of the genetic basis of yield-related traits and could be used for crop improvement in B. napus.
摘要：
油菜（甘蓝型油菜），第三大油料作物,是世界植物油和生物燃料的重要来源。虽然育种和产量得到了提高，与其他主要作物相比，油菜的产量仍然最低。因此，提高油菜籽产量对于植物油和优质蛋白质的高需求至关重要。每个工厂的Silique编号（SN），每荚种子(SP)，1000粒重（SW）是影响油菜种子产量的三个重要因素。一些产量相关性状，包括植物高度（PH），开花时间（FT），初枝数（BN）和每花序角果数（SI）也会影响单株产量（YP）。使用六种多基因座全基因组关联研究(ML-GWAS)方法，基于全基因组测序,在由403个油菜籽核心种质组成的小组中,共鉴定出908个产量相关的数量性状核苷酸(QTNs).ML-GWAS与转录组分析的整合，79个候选基因，包括BnaA09g39790D（RNA解旋酶），BnaA09g39950D（脂肪酶）和BnaC09g25980D（SWEET7），进一步鉴定，并通过qRT-PCR验证了12个基因对油菜中的SW或SP的影响。在20个优质油菜品种中，来自19个稳定QTNs的优良等位基因的分布表明，高产品种包含更多的优良等位基因。这些结果将有助于进一步了解产量相关性状的遗传基础，并可用于油菜的作物改良。
