PDF(Pubmed)
Abstract：
The production of recombinant proteins is one of the most significant achievements of biotechnology in the last century. These proteins are produced in the eukaryotic or prokaryotic heterologous hosts. By increasing the omics data especially related to different heterologous hosts as well as the presence of new amenable genetic engineering tools, we can artificially engineer heterologous hosts to produce recombinant proteins in sufficient quantities. Numerous recombinant proteins have been produced and applied in various industries, and the global recombinant proteins market size is expected to be cast to reach USD 2.4 billion by 2027. Therefore, identifying the weakness and strengths of heterologous hosts is critical to optimize the large-scale biosynthesis of recombinant proteins. E. coli is one of the popular hosts to produce recombinant proteins. Scientists reported some bottlenecks in this host, and due to the increasing demand for the production of recombinant proteins, there is an urgent need to improve this host. In this review, we first provide general information about the E. coli host and compare it with other hosts. In the next step, we describe the factors involved in the expression of the recombinant proteins in E. coli. Successful expression of recombinant proteins in E. coli requires a complete elucidation of these factors. Here, the characteristics of each factor will be fully described, and this information can help to improve the heterologous expression of recombinant proteins in E. coli.
摘要：
重组蛋白的生产是上个世纪生物技术最重要的成就之一。这些蛋白质在真核或原核异源宿主中产生。通过增加尤其是与不同异源宿主相关的组学数据，以及新的可适应基因工程工具的存在，我们可以人工设计异源宿主来生产足够数量的重组蛋白。许多重组蛋白已被生产和应用于各种行业，预计到2027年，全球重组蛋白市场规模将达到24亿美元。因此,识别异源宿主的弱点和优势对于优化重组蛋白的大规模生物合成至关重要。大肠杆菌是产生重组蛋白的流行宿主之一。科学家报告了这个宿主的一些瓶颈，由于对重组蛋白生产的需求不断增加，迫切需要改进这个主机。在这次审查中,我们首先提供有关大肠杆菌宿主的一般信息，并将其与其他宿主进行比较。下一步,我们描述了参与重组蛋白在大肠杆菌中表达的因素。重组蛋白在大肠杆菌中的成功表达需要对这些因素的完全阐明。这里,将充分描述每个因素的特征，这些信息有助于提高重组蛋白在大肠杆菌中的异源表达。
