Abstract：
The effect of helminthic infections on allergic diseases and asthma is still inconclusive. Moreover, there is considerable evidence suggesting that nitric oxide (NO), metalloproteinases and pro-inflammatory cytokines play a significant role in the physiopathology of these diseases. In this sense, the aim of our study is to investigate the ex vivo immunomodulatory effect of the laminated layer (LL, outside layer of parasitic cyst) of the helminth Echinococcus granulosus on NO, IL-17A and IL-10 production. In the first step of our study, we evaluated in vivo the NO, MMP-9, IL-17A, IL-10 levels in Algerian patients with allergic asthma and allergic rhinitis and their changes in relation with exacerbation status of the patients. In the principal part of our work, we assessed NO, IL-10 and IL-17A levels in supernatants of patients PBMC cultures before and after stimulation with LL. Our results indicate a significant reduction in NO production by PBMC of patients with allergic rhinitis and allergic asthma whether mild, moderate or severe after stimulation with LL. Interestingly, LL induces a significant decrease in the production of NO and IL17-A levels as well as an increase in the production of IL-10 in the cultures performed with PBMC of patients with severe allergic asthma. Importantly, our data indicate that LL exert a down-modulatory effect on inflammatory mediators (NO, IL-17A) and up immune-regulatory effect on IL-10 production. Collectively, our study supports the hygiene hypothesis suggesting that Echinococcus granulosus infection like other helminths could prevent and/or modulate inflammation responses during inflammatory diseases.
摘要：
蠕虫感染对过敏性疾病和哮喘的影响仍不确定。此外，有大量证据表明一氧化氮(NO)，金属蛋白酶和促炎细胞因子在这些疾病的病理生理学中起重要作用。在这个意义上,我们研究的目的是研究层压层的离体免疫调节作用(LL，寄生虫囊肿的外层）在NO上，IL-17A和IL-10的产生。在我们研究的第一步，我们在体内评估了NO，MMP-9,IL-17A,阿尔及利亚过敏性哮喘和过敏性鼻炎患者IL-10水平及其与患者病情加重的关系.在我们工作的主要部分，我们评估NO,在用LL刺激之前和之后,患者PBMC培养物上清液中的IL-10和IL-17A水平。我们的结果表明，无论是轻度的过敏性鼻炎和过敏性哮喘患者的PBMC产生的NO显著减少，用LL刺激后中度或重度。有趣的是,在用严重过敏性哮喘患者的PBMC进行的培养中，LL诱导NO和IL17-A水平的产生显着降低，以及IL-10的产生增加。重要的是,我们的数据表明LL对炎症介质(NO，IL-17A）和对IL-10产生的上调免疫调节作用。总的来说，我们的研究支持以下卫生学假说:细粒棘球蚴感染与其他蠕虫一样,可以预防和/或调节炎症性疾病期间的炎症反应.
