Abstract：
The ability to modulate the host immune response has allowed some parasites to establish themselves in the tissues of an immunocompetent organism. While some parasite excretion/secretion products (ESPs) were recently reported to induce differentiation of regulatory T cells (Tregs), their identity is not known. This work is aimed to identify and characterize ESPs of Taenia crassiceps cysticerci linked with Treg induction in vivo. ESPs were obtained from cultures of T. crassiceps cysticerci and inoculated in mice, measuring Treg levels by flow cytometry. Proteins in ESPs were analyzed by electrophoresis; then, ESPs were classified as either differential or conserved. Differentially included proteins were MS-sequenced and functionally characterized. Only 4 of 10 ESPs induced Tregs. Proteins with catalytic activity and those involved in immunological processes predominated, supporting the idea that these molecules could play an important role in the induction of Tregs.
摘要：
调节宿主免疫应答的能力已经允许一些寄生虫在免疫活性生物体的组织中建立自己。虽然最近报道了一些寄生虫排泄/分泌产物（ESP）诱导调节性T细胞（Tregs）的分化，他们的身份是未知的。这项工作的目的是在体内鉴定和表征与Treg诱导相关的足带虫囊虫的ESPs。从囊尾蚴的培养物中获得ESPs，并在小鼠中接种。通过流式细胞术测量Treg水平。通过电泳分析ESP中的蛋白质；然后，ESP分为差异或保守。对差异包含的蛋白质进行MS测序并进行功能表征。10个ESP中只有4个诱导了Tregs。具有催化活性和参与免疫过程的蛋白质占主导地位，支持这些分子可以在Tregs的诱导中发挥重要作用的观点。
