PDF(Pubmed)
Abstract：
Several studies have indicated that increased levels of insulin-like growth factor 1 (IGF-I) or altered levels of its binding proteins are associated with an increased risk of the most common cancers, including cancers of the colorectal, lung, breast and prostate. The aim of this study is to investigate the expression of IGF-1 in both calcifying epithelial odontogenic tumor (CEOT) and ameloblastoma.
UNASSIGNED: The research sample consisted of 23 paraffin block from Oral Pathology Department, Faculty of Dentistry, Damascus University, including six biopsies of CEOT, two biopsies of the plexiform ameloblastoma and 14 biopsies of the follicular ameloblastoma. All specimen were prepared and immunostained with rabbit polyclonal antibody to IGF-1. Immunostaining was assessed according to German-semiquantitative scoring system, and then the results were aggregated and statistically analyzed using SPSS version 13.0 (Student\'s test for two independent samples, one-way analysis of variance, Kruskal-Wallis test and Mann-Whitney U-test), the significance level P value less than 0.05 was considered statistically significant.
UNASSIGNED: All CEOT and ameloblastoma samples were positive for IGF-1 staining, except one sample of ameloblastoma, which was negative. The results showed that there were no statistically significant differences between CEOT and ameloblastoma in the values of IGF-1 expression (P=0.993) and IGF-1 expression rates (P=0.874) and in the frequency of IGF-1 expression (P=0.761) and IGF-1 staining intensity score (P=0.731).
UNASSIGNED: IGF-1 plays an important role in the growth of odontogenic tumors and there are no differences in IGF-1 expression between CEOT and ameloblastoma.
UNASSIGNED: The research sample consisted of 23 paraffin block from Oral Pathology Department, Faculty of Dentistry, Damascus University, including six biopsies of CEOT, two biopsies of the plexiform ameloblastoma and 14 biopsies of the follicular ameloblastoma. All specimen were prepared and immunostained with rabbit polyclonal antibody to IGF-1. Immunostaining was assessed according to German-semiquantitative scoring system, and then the results were aggregated and statistically analyzed using SPSS version 13.0 (Student\'s test for two independent samples, one-way analysis of variance, Kruskal-Wallis test and Mann-Whitney U-test), the significance level P value less than 0.05 was considered statistically significant.
UNASSIGNED: All CEOT and ameloblastoma samples were positive for IGF-1 staining, except one sample of ameloblastoma, which was negative. The results showed that there were no statistically significant differences between CEOT and ameloblastoma in the values of IGF-1 expression (P=0.993) and IGF-1 expression rates (P=0.874) and in the frequency of IGF-1 expression (P=0.761) and IGF-1 staining intensity score (P=0.731).
UNASSIGNED: IGF-1 plays an important role in the growth of odontogenic tumors and there are no differences in IGF-1 expression between CEOT and ameloblastoma.
摘要：
多项研究表明，胰岛素样生长因子1（IGF-I）水平的升高或其结合蛋白水平的改变与最常见癌症的风险增加有关。包括结直肠癌，肺，乳房和前列腺。这项研究的目的是研究IGF-1在钙化上皮牙源性肿瘤（CEOT）和成釉细胞瘤中的表达。
■研究样本由来自口腔病理科的23块石蜡组成，牙科学院,大马士革大学,包括六个CEOT活检，对丛状成釉细胞瘤进行了两次活检，对滤泡性成釉细胞瘤进行了14次活检。制备所有标本并用兔IGF-1多克隆抗体进行免疫染色。根据德国半定量评分系统评估免疫染色，然后使用SPSS13.0版对结果进行汇总和统计分析(对两个独立样本进行学生检验，单向方差分析，Kruskal-Wallis检验和Mann-WhitneyU检验)，显著性水平P值小于0.05被认为具有统计学意义.
■所有CEOT和成釉细胞瘤样本的IGF-1染色呈阳性，除了一个成釉细胞瘤样本,这是负面的。结果显示，CEOT和成釉细胞瘤之间的IGF-1表达值（P=0.993）和IGF-1表达率（P=0.874），IGF-1表达频率（P=0.761）和IGF-1染色强度评分（P=0.731）均无统计学差异。
■IGF-1在牙源性肿瘤的生长中起重要作用，CEOT和成釉细胞瘤之间的IGF-1表达没有差异。
■研究样本由来自口腔病理科的23块石蜡组成，牙科学院,大马士革大学,包括六个CEOT活检，对丛状成釉细胞瘤进行了两次活检，对滤泡性成釉细胞瘤进行了14次活检。制备所有标本并用兔IGF-1多克隆抗体进行免疫染色。根据德国半定量评分系统评估免疫染色，然后使用SPSS13.0版对结果进行汇总和统计分析(对两个独立样本进行学生检验，单向方差分析，Kruskal-Wallis检验和Mann-WhitneyU检验)，显著性水平P值小于0.05被认为具有统计学意义.
■所有CEOT和成釉细胞瘤样本的IGF-1染色呈阳性，除了一个成釉细胞瘤样本,这是负面的。结果显示，CEOT和成釉细胞瘤之间的IGF-1表达值（P=0.993）和IGF-1表达率（P=0.874），IGF-1表达频率（P=0.761）和IGF-1染色强度评分（P=0.731）均无统计学差异。
■IGF-1在牙源性肿瘤的生长中起重要作用，CEOT和成釉细胞瘤之间的IGF-1表达没有差异。
