PDF(Pubmed)
Abstract：
Earlier, we demonstrated the co-circulation of genetically distinct non-rodent-borne hantaviruses, including Boginia virus (BOGV) in the Eurasian water shrew (Neomys fodiens), Seewis virus (SWSV) in the Eurasian common shrew (Sorex araneus) and Nova virus (NVAV) in the European mole (Talpa europaea), in central Poland. To further investigate the phylogeny of hantaviruses harbored by soricid and talpid reservoir hosts, we analyzed RNAlater®-preserved lung tissues from 320 shrews and 26 moles, both captured during 1990-2017 across Poland, and 10 European moles from Ukraine for hantavirus RNA through RT-PCR and DNA sequencing. SWSV and Altai virus (ALTV) were detected in Sorex araneus and Sorex minutus in Boginia and the Białowieża Forest, respectively, and NVAV was detected in Talpa europaea in Huta Dłutowska, Poland, and in Lviv, Ukraine. Phylogenetic analyses using maximum-likelihood and Bayesian methods showed geography-specific lineages of SWSV in Poland and elsewhere in Eurasia and of NVAV in Poland and Ukraine. The ATLV strain in Sorex minutus from the Białowieża Forest on the Polish-Belarusian border was distantly related to the ATLV strain previously reported in Sorex minutus from Chmiel in southeastern Poland. Overall, the gene phylogenies found support long-standing host-specific adaptation.
摘要：
早些时候，我们证明了遗传上不同的非啮齿动物传播汉坦病毒的共同循环，包括欧亚水sh（Neomysfodiens）中的Boginia病毒（BOGV），欧亚普通sh（Sorexaraneus）中的Seewis病毒（SWSV）和欧洲痣（Talpaeuropaea）中的Nova病毒（NVAV），在波兰中部。为了进一步研究由soricid和牛脂库宿主携带的汉坦病毒的系统发育，我们分析了RNAlater®-保存的肺组织从320和26痣，两者都在1990-2017年期间在波兰被捕，和来自乌克兰的10个欧洲痣通过RT-PCR和DNA测序获得汉坦病毒RNA。在Boginia和Biaswowieça森林的Sorexaraneus和Sorexminutus中检测到SWSV和Altai病毒（ALTV），分别,并且在胡塔·德托夫斯卡的Talpaeuropaea中检测到NVAV，波兰,在利沃夫,乌克兰。使用最大似然和贝叶斯方法进行的系统发育分析显示，波兰和欧亚大陆其他地区的SWSV以及波兰和乌克兰的NVAV的地理特定谱系。来自波兰-白俄罗斯边界的Biaswowieāa森林的Sorexminutus中的ATLV菌株与先前在波兰东南部Chmiel的Sorexminutus中报道的ATLV菌株密切相关。总的来说,发现的基因系统发育支持长期的宿主特异性适应。
