PDF(Pubmed)
Abstract：
OBJECTIVE: Osteoarthritis (OA) is an age-related joint disease that involves the degeneration of cartilage and is the most prevalent form of arthritis, affecting a large part of the population. OA is a multifactorial disorder, and no single etiological mechanism has been found to be common to all forms of the disease. Currently used therapies for control of the disease are mainly nonsteroidal anti-inflammatory drugs (NSAIDs) and corticosteroid medications. The aim of this study was to investigate the extract from Crocus sativus as a biological disease-suppressing therapy agent.
METHODS: Balb/c mice were injected intra-articularly with Clostridium histolyticum type IA for induction of osteoarthritis. The mice were randomized to five groups: control group, I group (CIOA untreated), II group (CIOA + 100 mg/kg/daily saffron), III group (CIOA + 50 mg/kg/daily saffron), IV group (CIOA + 25 mg/kg/daily saffron). Flow-cytometry analysis was used to study the splenocytes\' phenotype isolated from the treated animals. The serum levels of inflammatory and anti-inflammatory cytokines were analyzed with ELISA. The histological assessment was used to analyze the saffron extract effect on histopathological alterations.
RESULTS: Saffron treatment significantly decreased osteoarthritis-associated joint histological manifestations and decreased serum TNFα levels. The flow-cytometry analysis showed a decrease in pro-inflammatory immune cell subtypes in the spleen.
CONCLUSIONS: The results obtained suggest that saffron affected the disease progression and could be a potential therapeutic approach in osteoarthritic patients\' therapy.
METHODS: Balb/c mice were injected intra-articularly with Clostridium histolyticum type IA for induction of osteoarthritis. The mice were randomized to five groups: control group, I group (CIOA untreated), II group (CIOA + 100 mg/kg/daily saffron), III group (CIOA + 50 mg/kg/daily saffron), IV group (CIOA + 25 mg/kg/daily saffron). Flow-cytometry analysis was used to study the splenocytes\' phenotype isolated from the treated animals. The serum levels of inflammatory and anti-inflammatory cytokines were analyzed with ELISA. The histological assessment was used to analyze the saffron extract effect on histopathological alterations.
RESULTS: Saffron treatment significantly decreased osteoarthritis-associated joint histological manifestations and decreased serum TNFα levels. The flow-cytometry analysis showed a decrease in pro-inflammatory immune cell subtypes in the spleen.
CONCLUSIONS: The results obtained suggest that saffron affected the disease progression and could be a potential therapeutic approach in osteoarthritic patients\' therapy.
摘要：
目的：骨关节炎（OA）是一种与年龄有关的关节疾病，涉及软骨的退化，是关节炎的最常见形式，影响了很大一部分人口。OA是一种多因素疾病,并没有发现单一的病因机制对所有形式的疾病都是共同的。目前用于控制该疾病的疗法主要是非甾体抗炎药(NSAIDs)和皮质类固醇药物。本研究的目的是研究番红花提取物作为生物疾病抑制治疗剂。
方法：Balb/c小鼠关节内注射溶组织梭菌IA型以诱导骨关节炎。将小鼠随机分为5组:对照组,I组（CIOA未治疗），II组（CIOA+100mg/kg/日藏红花），III组（CIOA+50mg/kg/日藏红花），IV组(CIOA+25mg/kg/日藏红花)。流式细胞术分析用于研究从经处理的动物中分离的脾细胞表型。用ELISA分析血清炎性和抗炎性细胞因子的水平。组织学评估用于分析藏红花提取物对组织病理学改变的影响。
结果：藏红花治疗可显着减少骨关节炎相关的关节组织学表现，并降低血清TNFα水平。流式细胞术分析显示脾脏中促炎免疫细胞亚型的减少。
结论：获得的结果表明藏红花影响疾病进展，可能是骨关节炎患者治疗的潜在治疗方法。
方法：Balb/c小鼠关节内注射溶组织梭菌IA型以诱导骨关节炎。将小鼠随机分为5组:对照组,I组（CIOA未治疗），II组（CIOA+100mg/kg/日藏红花），III组（CIOA+50mg/kg/日藏红花），IV组(CIOA+25mg/kg/日藏红花)。流式细胞术分析用于研究从经处理的动物中分离的脾细胞表型。用ELISA分析血清炎性和抗炎性细胞因子的水平。组织学评估用于分析藏红花提取物对组织病理学改变的影响。
结果：藏红花治疗可显着减少骨关节炎相关的关节组织学表现，并降低血清TNFα水平。流式细胞术分析显示脾脏中促炎免疫细胞亚型的减少。
结论：获得的结果表明藏红花影响疾病进展，可能是骨关节炎患者治疗的潜在治疗方法。
