METHODS: Balb/c mice were injected intra-articularly with Clostridium histolyticum type IA for induction of osteoarthritis. The mice were randomized to five groups: control group, I group (CIOA untreated), II group (CIOA + 100 mg/kg/daily saffron), III group (CIOA + 50 mg/kg/daily saffron), IV group (CIOA + 25 mg/kg/daily saffron). Flow-cytometry analysis was used to study the splenocytes\' phenotype isolated from the treated animals. The serum levels of inflammatory and anti-inflammatory cytokines were analyzed with ELISA. The histological assessment was used to analyze the saffron extract effect on histopathological alterations.
RESULTS: Saffron treatment significantly decreased osteoarthritis-associated joint histological manifestations and decreased serum TNFα levels. The flow-cytometry analysis showed a decrease in pro-inflammatory immune cell subtypes in the spleen.
CONCLUSIONS: The results obtained suggest that saffron affected the disease progression and could be a potential therapeutic approach in osteoarthritic patients\' therapy.
方法:Balb/c小鼠关节内注射溶组织梭菌IA型以诱导骨关节炎。将小鼠随机分为5组:对照组,I组(CIOA未治疗),II组(CIOA+100mg/kg/日藏红花),III组(CIOA+50mg/kg/日藏红花),IV组(CIOA+25mg/kg/日藏红花)。流式细胞术分析用于研究从经处理的动物中分离的脾细胞表型。用ELISA分析血清炎性和抗炎性细胞因子的水平。组织学评估用于分析藏红花提取物对组织病理学改变的影响。
结果:藏红花治疗可显着减少骨关节炎相关的关节组织学表现,并降低血清TNFα水平。流式细胞术分析显示脾脏中促炎免疫细胞亚型的减少。
结论:获得的结果表明藏红花影响疾病进展,可能是骨关节炎患者治疗的潜在治疗方法。