PDF(Pubmed)
Abstract：
The Notch signalling pathway is one of the most conserved and well-characterised pathways involved in cell fate decisions and the development of many diseases, including cancer. Among them, it is worth noting the Notch4 receptor and its clinical application, which may have prognostic value in patients with colon adenocarcinoma. The study was performed on 129 colon adenocarcinomas. Immunohistochemical and fluorescence expression of Notch4 was performed using the Notch4 antibody. The associations between the IHC expression of Notch4 and clinical parameters were analysed using the Chi2 test or Chi2Yatesa test. The Kaplan-Meier analysis and the log-rank test were used to verify the relationship between the intensity of Notch4 expression and the 5-year survival rate of patients. Intracellular localisation of Notch4 was detected by the use of the immunogold labelling method and TEM. 101 (78.29%) samples had strong Notch4 protein expression, and 28 (21.71%) samples were characterised by low expression. The high expression of Notch4 was clearly correlated with the histological grade of the tumour (p < 0.001), PCNA immunohistochemical expression (p < 0.001), depth of invasion (p < 0.001) and angioinvasion (p < 0.001). We can conclude that high expression of Notch4 is correlated with poor prognosis of colon adenocarcinoma patients (log-rank, p < 0.001).
摘要：
Notch信号通路是涉及细胞命运决定和许多疾病发展的最保守和特征明确的通路之一。包括癌症.其中,值得注意的是Notch4受体及其临床应用,这可能对结肠腺癌患者具有预后价值。该研究对129例结肠腺癌进行。使用Notch4抗体进行Notch4的免疫组织化学和荧光表达。使用Chi2检验或Chi2Yatesa检验分析Notch4的IHC表达与临床参数之间的关联。采用Kaplan-Meier分析和log-rank检验验证Notch4表达强度与患者5年生存率的关系。通过使用免疫金标记方法和TEM检测Notch4的细胞内定位。101例(78.29%)样本有较强的Notch4蛋白表达,和28（21.71％）样品的特征是低表达。Notch4的高表达与肿瘤的组织学分级密切相关(p<0.001)。PCNA免疫组化表达（p<0.001），侵袭深度（p<0.001）和血管侵袭（p<0.001）。我们可以得出结论，Notch4的高表达与结肠腺癌患者的不良预后相关（log-rank，p<0.001)。
