PDF(Pubmed)
Abstract：
Vitamin B6 is an indispensable micronutrient in organisms and is widely distributed in blood, tissues, and organs. Changes in the content and ratio of vitamin B6 can affect the entire physiological condition of the body, so it becomes particularly important to reveal the relationship between changes in its content and disease by monitoring vitamin B6 levels in the organism. In this study, a two-dimensional liquid chromatography-UV detector (2D-LC-UV) was used to establish a method for the simultaneous detection of PLP, PA, and PL for the first time. First, PLP, PA, and PL were extracted with plasma: 0.6 M TCA: ultrapure water = 1:2:3 (v/v/v) and then derivatized. Enrichment and preliminary separation were performed on a one-dimensional column and automatically entered into a two-dimensional column for further separation. This method exhibited good selectivity, and the correlation coefficients for the analyte calibration curves were >0.99. The detection limits for PLP, PA, and PL were 0.1, 0.2, and 4 nmol/L, respectively. The results showed that the system has high loading capacity, excellent resolution, and a good peak shape. This method is expected to provide applicability for the determination of PLP, PA, and PL in pharmacological, pharmaceutical, and clinical research.
摘要：
维生素B6是生物体内不可缺少的微量营养素,广泛分布于血液中,组织，和器官。维生素B6的含量和比例的变化可以影响身体的整个生理状况，因此，通过监测生物体中维生素B6的水平来揭示其含量变化与疾病之间的关系变得尤为重要。在这项研究中,利用二维液相色谱-紫外检测器(2D-LC-UV)建立了PLP的同时检测方法,PA,和PL第一次。首先,PLP,PA,和PL用血浆：0.6MTCA：超纯水=1：2：3（v/v/v）提取，然后衍生化。在一维柱上进行富集和初步分离，并自动进入二维柱中进行进一步分离。该方法具有良好的选择性,分析物校准曲线的相关系数>0.99。PLP的检测限,PA,和PL分别为0.1、0.2和4nmol/L，分别。结果表明,该系统具有较高的承载能力,出色的分辨率,和良好的峰形。该方法有望为PLP的测定提供适用性。PA,和PL在药理学上，Pharmaceutical,和临床研究。
