Abstract：
Molecular scale nanopatterns of bioactive molecules have been used to study the effect of transmembrane receptor arrangement on a variety of cell types, including immune cells and their immune response in particular. However, state-of-the-art fabrication approaches have thus far enabled the production of patterns with control over one receptor type only. Herein, we describe a protocol to fabricate arrays for the molecular scale control of the segregation between activating and inhibitory receptors in NK cells. We used this platform to study how ligand segregation regulates NK cell inhibitory signaling and function. The arrays are based on patterns of nanodots of two metals, selectively functionalized with activating and inhibitory ligands. Due to the versatility of our functionalization approach, this protocol can be applied to configurate virtually any combination of extracellular ligands into controlled multifunctional arrays.
摘要：
生物活性分子的分子尺度纳米模式已用于研究跨膜受体排列对多种细胞类型的影响，包括免疫细胞和它们的免疫反应。然而,到目前为止，最先进的制造方法已经能够生产只控制一种受体类型的模式。在这里，我们描述了一种方案，以制造用于NK细胞中激活和抑制性受体之间分离的分子尺度控制的阵列。我们使用这个平台来研究配体分离如何调节NK细胞抑制信号和功能。阵列基于两种金属的纳米点的图案，用活化和抑制配体选择性官能化。由于我们功能化方法的多功能性，该方案可用于将细胞外配体的任何组合配置为受控的多功能阵列。
