Abstract：
Fowl adenovirus serotype 4 (FAdV-4) is a double-stranded DNA virus that mainly infects broiler chickens and has caused huge economic losses to the poultry industry. Recently, an FAdV-4 strain, SDLC202009, the causative pathogen of hydropericardium-hepatitis syndrome (HHS) in Liaocheng, Shandong, was isolated from commercial laying hens and propagated in specific pathogen free SPF chicken embryos. Pathogenicity studies showed that SDLC202009 could infect SPF chicken embryos and chickens, with a mortality rate of 100%. The complete genome was sequenced, and phylogenetic analysis showed that SDLC202009 belonged to the FAdV-4 cluster, with a genome length of 43, 077 bp. The SDLC202009 had 99.9% identity with the JSJ13 and SD1601, which were recently isolated in China. Compared to the recently isolated strain in China, SDLC202009 had deleted open reading frame 19 (ORF19), ORF27, ORF48, and ORF0. SDLC202009 harbored amino acid site mutations in the main structural proteins hexon, fiber1, and fiber2 similar with those in highly pathogenic strains. Furthermore, SDLC202009 showed unique mutations in hexon A571P, fiber1 E216K, and fiber2 N98K. In summary, our findings provide theoretical support for prevention and control of the HHS.
摘要：
禽腺病毒血清型4(FAdV-4)是一种主要感染肉鸡的双链DNA病毒,给养禽业造成了巨大的经济损失。最近,一种FAdV-4菌株,SDLC202009,聊城市心包积水型肝炎综合征(HHS)病原体,山东,从商业产蛋鸡中分离并在无特定病原体的SPF鸡胚胎中繁殖。致病性研究表明,SDLC202009可以感染SPF鸡胚和鸡,死亡率为100%。完整的基因组被测序，系统发育分析表明，SDLC202009属于FAdV-4簇，基因组长度为43,077bp。SDLC202009与最近在中国分离的JSJ13和SD1601具有99.9%的同一性。与中国最近分离的菌株相比，SDLC202009删除了开放阅读框19(ORF19)，ORF27、ORF48和ORF0。SDLC202009在主要结构蛋白六邻体中含有氨基酸位点突变，纤维1和纤维2与高致病性菌株相似。此外，SDLC202009在六邻体A571P中显示出独特的突变，纤维1E216K,和纤维2N98K。总之,我们的研究结果为HHS的预防和控制提供了理论支持。
