Overexpression of Global Regulator SCrp Leads to the Discovery of New Angucyclines in Streptomyces sp. XS-16.

全球调节因子 SCrp 的过表达导致在链霉菌属中发现新的 Angucycles 。XS - 16.

• 影响指数: 6.085
• 发表时间: 2023 Apr 14
• 来源期刊: Mar Drugs
• DOI: 10.3390/md21040240
• PMID: 37103379
• 文章类型: Journal Article
• 中科院分区: Q1
• 方向: 医学
• 作者列表: Xu X,Zhang F,Zhou L,Chang Y,Che Q,Zhu T,Li D,Zhang G

关键词: SCrp; Streptomyces sp.; angucyclines; global regulator; overexpression

Mesh: Antineoplastic Agents / chemistry; Streptomyces / metabolism; Cell Line, Tumor; Magnetic Resonance Spectroscopy; Molecular Structure

Abstract

Six angucyclines including three unreported compounds (1-3) were isolated from Streptomyces sp. XS-16 by overexpressing the native global regulator of SCrp (cyclic AMP receptor). The structures were characterized based on nuclear magnetic resonance (NMR) and spectrometry analysis and assisted by electronic circular dichroism (ECD) calculations. All compounds were tested for their antitumor and antimicrobial activities, and compound 1 showed different inhibitory activities against various tumor cell lines with IC50 values ranging from 0.32 to 5.33 μM.

摘要

从链霉菌属中分离出包括三个未报道的化合物（1-3）的六个angucycles。XS-16通过过表达SCrp(环状AMP受体)的天然全局调节因子。基于核磁共振（NMR）和光谱分析并通过电子圆二色性（ECD）计算对结构进行了表征。测试了所有化合物的抗肿瘤和抗菌活性。和化合物1对各种肿瘤细胞系表现出不同的抑制活性,IC50值为0.32至5.33μM。