Abstract：
Eucommiae Folium (Duzhongye) is a traditional Chinese medicine with a long history of use in China. However, its quality-marker in Chinese Pharmacopoeia is poorly defined nowadays. The study, therefore, conducted an ultra-high-performance liquid chromatography coupled with hybrid quadrupole-orbitrap tandem mass spectrometry analysis to obtain accurate data. The obtained data were then compared with the authentic standards library using Xcalibur 4.1 software package and TraceFinder General Quan. Through the comparison, the study has putatively identified 26 bioactive compounds, which include 17 flavonoid derivatives (catechin, quercetin 3-gentiobioside, quercetin 3-O-β-D-glucose-7-O-β-D-gentiobioside, taxifolin, myricetin 3-O-galactoside, myricitrin, hyperoside, rutin, isoquercitrin, quercetin 3-O-β-xylopyranoside, quercitrin, isorhamnetin 3-O-β-D-glucoside, quercetin, kaempferol, S-eriodictyol, S-naringenin, and phloridzin), four caffeoylquinic acids (neochlorogenic acid, chlorogenic acid, isochlorogenic acid A, and isochlorogenic acid C), two alkaloids (vincamine and jervine), one lignan (pinoresinol), one xanthone (cowaxanthone B), and one steroid (cholesteryl acetate). Of these, flavonoid isoquercitrin is recommended as the new and additional pharmacopeia quality-marker candidate, which can not only overcome the unreliability of old quality-marker but also recognize the possible counterfeit.
摘要：
杜仲叶是我国使用历史悠久的传统中药。然而,目前《中国药典》对其质量标记(Q-marker)定义不明确。因此，该研究进行了超高效液相色谱与混合四极杆-轨道阱串联质谱（UHPLC-四极杆-Exactive-OrbitrapMS/MS）分析，以获得准确的数据。然后使用Xcalibur4.1软件包和TraceFinderGeneralQuan将获得的数据与真实的标准库进行比较。通过比较,该研究已经确定了26种生物活性化合物，其中包括17种类黄酮衍生物（儿茶素，槲皮素3-龙胆苷，槲皮素3-O-β-D-葡萄糖-7-O-β-D-龙胆苷,taxifolin,杨梅素3-O-半乳糖苷，Myricitrin,金丝桃苷,芦丁,异槲皮苷,槲皮素3-O-β-吡喃木糖苷，槲皮苷,异鼠李素3-O-β-D-葡萄糖苷，槲皮素,山奈酚,S-紫二酚,S-柚皮素,和phoridzin)，四种咖啡酰基奎尼酸(新绿原酸，绿原酸,异绿原酸A,和异绿原酸C)，两种生物碱（长春碱和肉桂），一种木脂素（松脂醇），一个黄吨酮（黄吨酮B），和一种类固醇（胆固醇乙酸酯）。其中,黄酮类异槲皮苷被推荐为新的和额外的药典Q标记候选物，这不仅可以克服旧Q标记的不可靠性，而且还可以识别可能的伪造。本文受版权保护。保留所有权利。
