Abstract：
With the aim of monocrotophos pesticides detection in environmental and food samples at point-of-care (PoC) application, this research, for the first time, explores silica alcogel as an immobilization matrix to support the development of in-house customized nano-enabled \"chromagrid-lighbox\" as a sensing system. This system is fabricated using laboratory waste materials and demonstrates the detection of highly hazardous monocrotophos pesticide using a smartphone. Nano-enabled chromagrid is a chip-like assembly filled with silica alcogel -a nanomaterial (hence the name \"nano-enabled\" chromagrid), and \"chromogenic reagents\" which is required for the enzymatic detection of monocrotophos. Lightbox is the imaging station fabricated to provide constant lighting conditions to the chromagrid to capture accurate colorimetric data. The silica alcogel used in this system was synthesized from Tetraethyl orthosilicate (TEOS) via a sol-gel method and characterized using advanced analytical techniques. Further, three chromagrid assays were developed for the optical detection of monocrotophos with a low detection limit (LOD) at 0.421 ng ml-1 (by α-NAc chromagrid assay), 0.493 ng ml-1 (by DTNB chromagrid assay) and 0.811 ng ml-1 (by IDA chromagrid assay). The developed novel PoC chromagrid-lightbox system is capable of on-site detection of monocrotophos in environmental as well as food samples. This system is able to be manufacture prudently using recyclable waste plastic. Overall, such developed eco-friendly PoC testing system will surely manage rapid detection of monocrotophos pesticide needed for environmental and sustainable agricultural management.
摘要：
为了在即时护理（PoC）应用中检测环境和食品样品中的久效磷农药，这项研究,第一次,探索二氧化硅醇凝胶作为固定基质，以支持开发内部定制的纳米功能“chromagrid-lightbox”作为传感系统。该系统使用实验室废料制造，并演示使用智能手机检测高度危险的久效磷农药。纳米发色层是一种芯片状组件，填充有二氧化硅醇凝胶-一种纳米材料（因此称为“纳米发色层”），以及酶促检测久效磷所需的“显色剂”。Lightbox是成像站，可为色栅提供恒定的照明条件，以捕获准确的色度数据。该系统中使用的二氧化硅醇凝胶是通过溶胶-凝胶法由原硅酸四乙酯（TEOS）合成的，并使用先进的分析技术进行了表征。Further,开发了三种色栅测定法，用于光学检测久效磷的低检测限（LOD）为0.421ngml-1（通过α-NAc色栅测定法），0.493ngml-1（通过DTNB铬合金测定）和0.811ngml-1（通过IDA铬合金测定）。开发的新型PoC色栅-灯箱系统能够现场检测环境和食品样品中的久效磷。该系统能够使用可回收的废塑料谨慎地制造。总的来说,这种开发的生态友好型PoC测试系统必将管理环境和可持续农业管理所需的久效磷农药的快速检测。
