RESULTS: The structural and functional analysis of 30 hypothetical proteins was carried out in this research. Out of these, 3 hypothetical functions (Q8V547, Q8V4S4, Q8V4Q4) could be assigned a structure and function confidently. Q8V547 protein in Monkeypox virus Zaire-96-I-16 is predicted as an apoptosis regulator which promotes viral replication in the infected host cell. Q8V4S4 is predicted as a nuclease responsible for viral evasion in the host. The function of Q8V4Q4 is to prevent host NF-kappa-B activation in response to pro-inflammatory cytokines like TNF alpha or interleukin 1 beta.
CONCLUSIONS: Out of the 30 hypothetical proteins of Monkeypox virus Zaire-96-I-16, 3 were annotated using various bioinformatics tools. These proteins function as apoptosis regulators, nuclease, and inhibitors of NF-Kappa-B activator. The functional and structural annotation of the proteins can be used to perform a docking with potential leads to discover novel drugs and vaccines against the Monkeypox. In vivo research can be carried out to identify the complete potential of the annotated proteins.
结果:在这项研究中对30种假设蛋白质进行了结构和功能分析。在这些中,3个假设的功能(Q8V547,Q8V4S4,Q8V4Q4)可以放心地分配一个结构和功能。猴痘病毒Zaire-96-I-16中的Q8V547蛋白被预测为促进感染宿主细胞中病毒复制的凋亡调节因子。预测Q8V4S4是负责宿主中病毒逃避的核酸酶。Q8V4Q4的功能是防止宿主NF-kappa-B激活以响应促炎细胞因子如TNFα或白介素1β。
结论:在猴痘病毒扎伊尔-96-I-16的30种假设蛋白中,有3种使用各种生物信息学工具进行了注释。这些蛋白质作为细胞凋亡调节因子,核酸酶,和NF-κB激活剂的抑制剂。蛋白质的功能和结构注释可用于与潜在的线索进行对接,以发现针对猴痘的新药和疫苗。可以进行体内研究以鉴定注释蛋白质的完整潜力。